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PMID: 1385112 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The multidrug resistance and cystic fibrosis genes have complementary patterns of epithelial expression.

The EMBO journal ·Vol. 11 ·No. 12 ·1992-12-00 ·Pages 4291-303

Trezise AE, Romano PR, Gill DR, Hyde SC, Sepúlveda FV, Buchwald M, Higgins CF

Abstract

The cystic fibrosis gene product, CFTR, and the multidrug resistance P-glycoprotein (encoded by the MDR1 gene) are structurally related proteins and both are associated with epithelial chloride channel activities. We have compared their cell-specific expression in the rat by in situ hybridization. In all tissues examined the two genes were found to have complementary patterns of expression, demonstrating exquisite regulation in both cell-specific and temporal fashions. Additionally, a switch in expression from one gene to the other was observed in certain tissues. For example, expression in the intestine switches from CFTR to MDR1 as the cells migrate across the crypt-villus boundary. A switch from CFTR to MDR1 expression was also observed in the uterine epithelium upon pregnancy. These data suggest that CFTR and P-glycoprotein serve analogous roles in epithelial cells and provide additional evidence that P-glycoprotein has a physiological role in regulating epithelial cell volume. The patterns of expression suggest that the regulation of these two genes is coordinately controlled.

Related Genes
CFTR MDR1
MeSH Terms
ATP Binding Cassette Transporter, Subfamily B, Member 1 Animals Base Sequence Chloride Channels Cystic Fibrosis/genetics Cystic Fibrosis Transmembrane Conductance Regulator Drug Resistance/genetics Embryonic and Fetal Development/genetics Epithelium/metabolism Female Gene Expression Regulation In Situ Hybridization Intestinal Mucosa/metabolism Male Membrane Glycoproteins/genetics Membrane Proteins/genetics,metabolism Molecular Sequence Data Placenta/metabolism Pregnancy RNA Probes Rats Rats, Wistar Testis/metabolism Uterus/metabolism
Chemicals
ATP Binding Cassette Transporter, Subfamily B, Member 1 Chloride Channels Membrane Glycoproteins Membrane Proteins RNA Probes Cystic Fibrosis Transmembrane Conductance Regulator
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Trezise A E
Department of Genetics, Hospital for Sick Children, Toronto, Ontario, Canada.
Romano P R
Gill D R
Hyde S C
Sepúlveda F V
Buchwald M
Higgins C F
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-12-00
Pages
4291-303
Language
English
Region
England
NLM ID
8208664
PMCID
PMC557002
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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