Home LiteratureArticle Details
PMID: 1383161 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of a Borrelia burgdorferi dnaJ homolog.

Infection and immunity ·Vol. 60 ·No. 11 ·1992-11-00 ·Pages 4965-8

Anzola J, Luft BJ, Gorgone G, Peltz G

Abstract

The gene encoding a Borrelia burgdorferi DnaJ homolog, located immediately 3' of the hsp70 gene, was characterized. Although there is a single copy of the dnaJ gene on the spirochetal chromosome, two distinct dnaJ transcripts are detected in B. burgdorferi RNA. RNA blot analysis indicates that the dnaJ gene can be transcribed alone or as part of a larger transcript containing the hsp70 homolog.

Related Genes
dnaJ hsp70
MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Borrelia burgdorferi Group/genetics Genes, Bacterial Heat-Shock Proteins/genetics Molecular Sequence Data RNA, Bacterial/genetics RNA, Messenger/genetics Sequence Alignment Transcription, Genetic
Chemicals
Bacterial Proteins Heat-Shock Proteins RNA, Bacterial RNA, Messenger
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Anzola J
Institute of Immunology and Biological Sciences, Syntex Research, Palo Alto, California 94303.
Luft B J
Gorgone G
Peltz G
References (24)
24 references, click to expand
  1. Chronic Lyme arthritis. Clinical and immunogenetic differentiation from rheumatoid arthritis.
    Ann Intern Med. 1979 Jun;90(6):896-901 PMID: 312615
  2. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  3. Heat shock proteins as antigens of bacterial and parasitic pathogens.
    Curr Top Microbiol Immunol. 1991;167:145-60 PMID: 1675978
  4. T cell and antibody reactivity with the Borrelia burgdorferi 60-kDa heat shock protein in Lyme arthritis.
    J Immunol. 1991 Jun 1;146(11):3985-92 PMID: 1709664
  5. Characterization of YDJ1: a yeast homologue of the bacterial dnaJ protein.
    J Cell Biol. 1991 Aug;114(4):609-21 PMID: 1869583
  6. Function of DnaJ and DnaK as chaperones in origin-specific DNA binding by RepA.
    Nature. 1991 Mar 14;350(6314):165-7 PMID: 2005967
  7. Analysis of T lymphocytes cloned from the synovial fluid and blood of a patient with Lyme arthritis.
    Int Immunol. 1990;2(11):1081-9 PMID: 2083228
  8. A self-splicing group I intron in the DNA polymerase gene of Bacillus subtilis bacteriophage SPO1.
    Cell. 1990 Oct 19;63(2):417-24 PMID: 2119891
  9. An ancient group I intron shared by eubacteria and chloroplasts.
    Science. 1990 Dec 14;250(4987):1570-3 PMID: 2125748
  10. Three Escherichia coli heat shock proteins are required for P1 plasmid DNA replication: formation of an active complex between E. coli DnaJ protein and the P1 initiator protein.
    Proc Natl Acad Sci U S A. 1990 Apr;87(7):2690-4 PMID: 2181445
  11. Stress proteins and immunology.
    Annu Rev Immunol. 1990;8:401-20 PMID: 2188668
  12. Phosphorylation of glutaminyl-tRNA synthetase and threonyl-tRNA synthetase by the gene products of dnaK and dnaJ in Escherichia coli K-12 cells.
    Biochimie. 1989 Sep-Oct;71(9-10):1079-87 PMID: 2512999
  13. A yeast gene important for protein assembly into the endoplasmic reticulum and the nucleus has homology to DnaJ, an Escherichia coli heat shock protein.
    J Cell Biol. 1989 Dec;109(6 Pt 1):2665-75 PMID: 2556404
  14. Stress proteins, infection, and immune surveillance.
    Cell. 1989 Oct 6;59(1):5-8 PMID: 2676194
  15. Nucleotide sequence of the Escherichia coli dnaJ gene and purification of the gene product.
    J Biol Chem. 1986 Feb 5;261(4):1778-81 PMID: 3003084
  16. The nucleotide sequence of the Escherichia coli K12 dnaJ+ gene. A gene that encodes a heat shock protein.
    J Biol Chem. 1986 Feb 5;261(4):1782-5 PMID: 3003085
  17. Organization of genes encoding two outer membrane proteins of the Lyme disease agent Borrelia burgdorferi within a single transcriptional unit.
    Infect Immun. 1986 Oct;54(1):207-12 PMID: 3019889
  18. A gene from Mycobacterium tuberculosis which is homologous to the DnaJ heat shock protein of E. coli.
    Nucleic Acids Res. 1988 Feb 25;16(4):1636 PMID: 3126487
  19. Escherichia coli heat shock gene mutants are defective in proteolysis.
    Genes Dev. 1988 Dec;2(12B):1851-8 PMID: 3149251
  20. Transcription termination and the regulation of gene expression.
    Annu Rev Biochem. 1986;55:339-72 PMID: 3527045
  21. The clinical evolution of Lyme arthritis.
    Ann Intern Med. 1987 Nov;107(5):725-31 PMID: 3662285
  22. Consensus sequence for Escherichia coli heat shock gene promoters.
    Proc Natl Acad Sci U S A. 1985 May;82(9):2679-83 PMID: 3887408
  23. A simple method for extraction of RNA from E. coli utilizing diethyl pyrocarbonate.
    Anal Biochem. 1970 Feb;33(2):459-63 PMID: 4910776
  24. Borrelia burgdorferi HSP70 homolog: characterization of an immunoreactive stress protein.
    Infect Immun. 1992 Sep;60(9):3704-13 PMID: 1379988
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.60.11.4965-4968.1992
Published
1992-11-00
Pages
4965-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258257
Subset
IM
Grants
NIAID NIH HHS · R01-AI32454-01 · United States
PHS HHS · U50/CCU206608-01 · United States
Databases
GENBANK
M63610, M63611, M63612, M63613, M63614, M63615, M63616, M63617, M97157, M97914
External Links
PubMed source DOI: 10.1128/iai.60.11.4965-4968.1992 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2