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PMID: 1382077 Published · ppublish English Journal Article

Identification of an E-selectin region critical for carbohydrate recognition and cell adhesion.

The Journal of cell biology ·Vol. 119 ·No. 1 ·1992-10-00 ·Pages 215-27

Erbe DV, Wolitzky BA, Presta LG, Norton CR, Ramos RJ, Burns DK, Rumberger JM, Rao BN, Foxall C, Brandley BK

Abstract

E-selectin elicits cell adhesion by binding to the cell surface carbohydrate, sialyl Lewis X (sLe(x)). We evaluated the effects of mutations in the E-selectin lectin domain on the binding of a panel of anti-E-selectin mAbs and on the recognition of immobilized sLe(x) glycolipid. Functional residues were then superimposed onto a three-dimensional model of the E-selectin lectin domain. This analysis demonstrated that the epitopes recognized by blocking mAbs map to a patch near the antiparallel beta sheet derived from the NH2 and COOH termini of the lectin domain and two adjacent loops. Mutations that affect sLe(x) binding map to this same region. These results thus define a small region of the E-selectin lectin domain that is critical for carbohydrate recognition.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal Binding Sites/physiology Cell Adhesion/physiology Cell Adhesion Molecules/chemistry,metabolism DNA Mutational Analysis E-Selectin Fluorescent Antibody Technique Humans Lewis X Antigen/chemistry,metabolism Membrane Glycoproteins/chemistry,metabolism Molecular Sequence Data Protein Conformation Rabbits Receptors, Immunologic/chemistry,metabolism Recombinant Fusion Proteins/metabolism
Chemicals
Antibodies, Monoclonal Cell Adhesion Molecules E-Selectin Lewis X Antigen Membrane Glycoproteins Receptors, Immunologic Recombinant Fusion Proteins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Erbe D V
Department of Immunology, Genentech, Inc., South San Francisco, California 94080.
Wolitzky B A
Presta L G
Norton C R
Ramos R J
Burns D K
Rumberger J M
Rao B N
Foxall C
Brandley B K
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.119.1.215
Published
1992-10-00
Pages
215-27
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289638
Subset
IM
Corrections
ErratumIn
-
External Links
PubMed source DOI: 10.1083/jcb.119.1.215 PMC Full Text View journal details
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