Abstract
Mycoplasma gallisepticum cell membranes were used to immunize mice to produce monoclonal antibodies to cell surface proteins. Three monoclonal antibodies were chosen for further characterization. All three reacted in immunoblots with an M. gallisepticum protein band of M(r) approximately 67,000 (designated pMGA). By using immunoelectron microscopy, pMGA was shown to be located on the cell surface. When M. gallisepticum whole cells were treated with up to 250 micrograms of trypsin per ml for 30 min, the only major protein lost from the cell surface as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by Western immunoblot transfer was pMGA. Two of the pMGA-specific monoclonal antibodies inhibited hemagglutination of chicken erythrocytes by M. gallisepticum S6, suggesting a role for pMGA in the attachment of M. gallisepticum to chicken erythrocytes. Sequencing the amino terminus of pMGA yielded 17 amino acids with no significant homology with the Mycoplasma pneumoniae attachment protein P1 or any other protein in the GenBank, Swiss-Prot, and EMBL data bases.
MeSH Terms
Amino Acid Sequence
Animals
Antibodies, Monoclonal/immunology
Bacterial Adhesion
Bacterial Proteins/analysis
Epitopes
Hemagglutinins/analysis,immunology
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Molecular Weight
Mycoplasma/chemistry
Chemicals
Antibodies, Monoclonal
Bacterial Proteins
Epitopes
Hemagglutinins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Markham P F
School of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia.
Glew M D
Brandon M R
Walker I D
Whithear K G
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