Viral DNA carried by human immunodeficiency virus type 1 virions.

Journal of virology ·Vol. 66 ·No. 8 ·1992-08-00 ·Pages 5067-74

Lori F, di Marzo Veronese F, de Vico AL, Lusso P, Reitz MS, Gallo RC

Abstract

A fundamental step in the replication of retroviruses is the reverse transcription of the viral RNA genome into a double-stranded DNA provirus. Retroviruses are believed to carry genomic information only as RNA, and synthesis of DNA is thought to start only after virus entry into the infected cell. We report here that infectious mature human immunodeficiency virus type 1 virions contain viral DNA of heterogeneous size. This heterogeneity seems to result from random stops of reverse transcription during minus- and plus-strand synthesis. The DNA carried by human immunodeficiency virus type 1 virions presumably originates from reverse transcription which takes place prior to or during formation of the mature virus particle.

MeSH Terms

Blotting, Southern Cell Line Centrifugation, Density Gradient DNA Probes DNA, Viral/genetics,isolation & purification HIV Reverse Transcriptase HIV-1/genetics,metabolism Humans Molecular Weight Polymerase Chain Reaction RNA-Directed DNA Polymerase/metabolism Restriction Mapping Virion/genetics,metabolism

Chemicals

DNA Probes DNA, Viral HIV Reverse Transcriptase RNA-Directed DNA Polymerase

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Lori F

Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, Maryland 20892.

di Marzo Veronese F

de Vico A L

Lusso P

Reitz M S

Gallo R C

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.66.8.5067-5074.1992

Published

1992-08-00

Pages

5067-74

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC241368

Subset

External Links

PubMed source DOI: 10.1128/JVI.66.8.5067-5074.1992 PMC Full Text View journal details

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