Assay of poly-beta-hydroxybutyric acid.

Journal of bacteriology ·Vol. 82 ·1961-07-00 ·Pages 33-6

LAW JH, SLEPECKY RA

Abstract

Law, John H. (Harvard University, Cambridge, Mass.) and Ralph A. Splepecky. Assay of poly-beta-hydroxybutyric acid. J. Bacteriol. 82:33-36. 1961-A convenient spectrophotometric assay of bacterial poly-beta-hydroxybutyric acid has been devised. Quantitative conversion of poly-beta-hydroxybutyric acid to crotonic acid by heating in concentrated sulfuric acid and determination of the ultraviolet absorption of the produce permits an accurate determination of this material in quantities down to 5 mug. This method has been used to follow the production of poly-beta-hydroxybutyric acid by Bacillus megaterium strain KM.

Keywords

BACILLUS/metabolism BUTYRATES/chemistry

MeSH Terms

Bacillus/metabolism Bacillus megaterium Butyrates/chemistry Crotonates Hydroxybutyrates Polyesters Sulfuric Acids

Chemicals

Butyrates Crotonates Hydroxybutyrates Polyesters Sulfuric Acids poly-beta-hydroxybutyrate crotonic acid

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

LAW J H

SLEPECKY R A

References (9)

9 references, click to expand

A group of pseudomonads able to synthesize poly-beta-hydroxybutyric acid.
Nature. 1959 May 30;183(4674):1538-9 PMID: 13666814
Synthesis and breakdown of poly-beta-hydroxybutyric acid by bacteria.
J Gen Microbiol. 1959 Jun;20(3):510-8 PMID: 13664898
Sudanophilic granules and lipid of Pseudomonas methanica.
J Bacteriol. 1960 Sep;80:321-4 PMID: 13750963
Poly-beta-hyroxybutyrate metabolism in washed suspensions of Bacillus cereus and Bacillus megaterium.
J Gen Microbiol. 1958 Aug;19(1):210-22 PMID: 13575769
Occurrence of poly-beta-hydroxybutyric acid in aerobic gram-negative bacteria.
Nature. 1958 Sep 20;182(4638):800-1 PMID: 13590114
THE ROLE OF ORGANIC SUBSTRATES IN BACTERIAL PHOTOSYNTHESIS.
Proc Natl Acad Sci U S A. 1959 Aug;45(8):1246-60 PMID: 16590501
The isolation and estimation of the poly-beta-hydroxybutyrate inclusions of Bacillus species.
J Gen Microbiol. 1958 Aug;19(1):198-209 PMID: 13575768
Characterization of the protoplasmic constituents of bacillus megaterium.
J Bacteriol. 1953 Dec;66(6):696-702 PMID: 13117796
Role of poly-beta-hydroxybutyric acid in the assimilation of organic carbon by bacteria.
Nature. 1959 May 23;183(4673):1440-2 PMID: 13657155

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.82.1.33-36.1961

Published

1961-07-00

Pages

33-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC279110

Subset

External Links

PubMed source DOI: 10.1128/jb.82.1.33-36.1961 PMC Full Text View journal details

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Assay of poly-beta-hydroxybutyric acid.

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