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PMID: 1375199 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Localization of two epitopes recognized by monoclonal antibody PCG-4 on Clostridium difficile toxin A.

Infection and immunity ·Vol. 60 ·No. 6 ·1992-06-00 ·Pages 2488-92

Frey SM, Wilkins TD

Abstract

The toxin A gene of Clostridium difficile contains a 2.5-kb region encoding a series of contiguous repeating units located at the COOH terminus of the molecule. We previously showed that the monoclonal antibody (MAb) PCG-4, which neutralizes the enterotoxic activity of toxin A, binds to epitopes located within these repeating units. In the present study, we subcloned a series of fragments from this portion of the gene. The recombinant peptides expressed from the gene fragments were examined for reactivity with MAb PCG-4 to identify the epitopes involved in binding. Our results showed that MAb PCG-4 recognizes epitopes in amino acid residues 2097 through 2141 and amino acid residues 2355 through 2398.

MeSH Terms
Amino Acid Sequence Antibodies, Monoclonal/immunology Bacterial Toxins/immunology Clostridioides difficile/metabolism Enterotoxins/immunology Epitopes/analysis Molecular Sequence Data Peptide Fragments/immunology
Chemicals
Antibodies, Monoclonal Bacterial Toxins Enterotoxins Epitopes Peptide Fragments tcdA protein, Clostridium difficile
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Frey S M
Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061.
Wilkins T D
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.60.6.2488-2492.1992
Published
1992-06-00
Pages
2488-92
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC257186
Subset
IM
Grants
NIAID NIH HHS · AI 15749 · United States
External Links
PubMed source DOI: 10.1128/iai.60.6.2488-2492.1992 PMC Full Text View journal details
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