Home LiteratureArticle Details
PMID: 1374586 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody.

The American journal of pathology ·Vol. 140 ·No. 5 ·1992-05-00 ·Pages 1025-30

Hameed A, Olsen KJ, Cheng L, Fox WM, Hruban RH, Podack ER

Abstract

Perforin is a potent cytolytic pore-forming protein expressed in cytoplasmic granules of cytotoxic T lymphocytes and natural killer cells. A new monoclonal antibody raised against human perforin was used to detect both in vitro and in vivo perforin expression in cytotoxic cells. Immunohistochemical analysis of human peripheral blood mononuclear cells cultured in recombinant interleukin-2 (rIL-2) showed strong granular cytoplasmic staining of the IL-2 activated cytotoxic cells. Fresh-frozen tissue sections from patients with heart allograft rejection were also stained. Strong granular cytoplasmic staining of the mononuclear inflammatory infiltrate characteristic for perforin in cardiac allograft rejection was observed. The detection and quantitative analysis of perforin-associated cytotoxic cells by the human anti-perforin monoclonal antibody will help to evaluate the significance of these functionally distinct cytotoxic cells in human tissue.

MeSH Terms
Antibodies, Monoclonal/immunology Antibody Specificity Biomarkers Humans Immunohistochemistry/methods Membrane Glycoproteins Membrane Proteins/immunology,metabolism Perforin Pore Forming Cytotoxic Proteins Staining and Labeling T-Lymphocytes, Cytotoxic/metabolism
Chemicals
Antibodies, Monoclonal Biomarkers Membrane Glycoproteins Membrane Proteins Pore Forming Cytotoxic Proteins Perforin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hameed A
Department of Pathology, Johns Hopkins Hospital, Baltimore, Maryland 21205.
Olsen K J
Cheng L
Fox W M
Hruban R H
Podack E R
References (33)
33 references, click to expand
  1. Perforin gene expression in granular lymphocyte proliferative disorders.
    Blood. 1990 Feb 1;75(3):704-8 PMID: 2297572
  2. A working formulation for the standardization of nomenclature in the diagnosis of heart and lung rejection: Heart Rejection Study Group. The International Society for Heart Transplantation.
    J Heart Transplant. 1990 Nov-Dec;9(6):587-93 PMID: 2277293
  3. Cytolysis by Ca-permeable transmembrane channels. Pore formation causes extensive DNA degradation and cell lysis.
    J Exp Med. 1989 Mar 1;169(3):765-77 PMID: 2538546
  4. Perforin and serine esterase gene expression in stimulated human T cells. Kinetics, mitogen requirements, and effects of cyclosporin A.
    J Exp Med. 1989 Dec 1;170(6):2105-18 PMID: 2584937
  5. Cytotoxic T lymphocyte mediated cytolysis.
    Biochemistry. 1988 Apr 19;27(8):2641-6 PMID: 3042021
  6. Identification and characterization of a pore-forming protein of human peripheral blood natural killer cells.
    J Exp Med. 1986 Dec 1;164(6):2061-76 PMID: 3097239
  7. Isolation and characterization of cytotoxic granules from human lymphokine (interleukin 2) activated killer cells.
    Cancer Res. 1988 Aug 15;48(16):4681-8 PMID: 3260817
  8. Characterization of granzymes A and B isolated from granules of cloned human cytotoxic T lymphocytes.
    J Immunol. 1988 Nov 15;141(10):3471-7 PMID: 3263427
  9. Inhibition of NK and ADCC activity by antibodies against purified cytoplasmic granules from rat LGL tumors.
    J Leukoc Biol. 1987 Dec;42(6):642-52 PMID: 3316462
  10. Structure and function of human perforin.
    Nature. 1988 Sep 29;335(6189):448-51 PMID: 3419519
  11. Specific release of proteoglycans from human natural killer cells during target lysis.
    Nature. 1985 Nov 21-27;318(6043):289-91 PMID: 4069203
  12. Cytotoxic T-lymphocytes. How do they function?
    Immunol Rev. 1983;72:5-42 PMID: 6347868
  13. Natural killer cells: their roles in defenses against disease.
    Science. 1981 Oct 2;214(4516):24-30 PMID: 7025208
  14. Interleukin 2 induction of pore-forming protein gene expression in human peripheral blood CD8+ T cells.
    J Exp Med. 1990 Apr 1;171(4):1269-81 PMID: 1691263
  15. Perforin expression in lymphocytes infiltrated to human colorectal cancer.
    Br J Cancer. 1991 Aug;64(2):239-42 PMID: 1892750
  16. Immunohistochemical localization of granzyme B antigen in cytotoxic cells in human tissues.
    Am J Pathol. 1991 May;138(5):1069-75 PMID: 2024702
  17. Constitutive expression of pore-forming protein in peripheral blood gamma/delta T cells: implication for their cytotoxic role in vivo.
    J Exp Med. 1990 Dec 1;172(6):1877-80 PMID: 2124255
  18. Perforin, a pore-forming protein detectable by monoclonal antibodies, is a functional marker for killer cells.
    Int Immunol. 1990;2(7):677-84 PMID: 2278998
  19. Isolation and biochemical and functional characterization of perforin 1 from cytolytic T-cell granules.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8629-33 PMID: 2417226
  20. TCGF (IL 2)-receptor inducing factor(s). I. Regulation of IL 2 receptor on a natural killer-like cell line (YT cells).
    J Immunol. 1985 Mar;134(3):1623-30 PMID: 2578514
  21. Cloning, analysis, and expression of murine perforin 1 cDNA, a component of cytolytic T-cell granules with homology to complement component C9.
    Proc Natl Acad Sci U S A. 1989 Jan;86(1):247-51 PMID: 2783486
  22. Multiple mechanisms of lymphocyte-mediated killing.
    Immunol Today. 1988 May;9(5):140-4 PMID: 3076768
  23. Characterization of three serine esterases isolated from human IL-2 activated killer cells.
    J Immunol. 1988 Nov 1;141(9):3142-7 PMID: 3262682
  24. The molecular basis of target cell killing by human lymphocytes and of killer cell self-protection.
    Immunol Rev. 1988 Mar;103:87-98 PMID: 3292398
  25. Mechanism of lymphocyte-mediated cytotoxicity.
    Annu Rev Immunol. 1985;3:31-58 PMID: 3904772
  26. Human natural killer cells: biologic and pathologic aspects.
    Lab Invest. 1984 May;50(5):489-513 PMID: 6201674
  27. Cytolytic activity of purified cytoplasmic granules from cytotoxic rat large granular lymphocyte tumors.
    J Exp Med. 1984 Jul 1;160(1):75-93 PMID: 6736872
  28. Perforin-mediated myocardial damage in acute myocarditis.
    Lancet. 1990 Oct 27;336(8722):1019-21 PMID: 1699101
  29. T-cell effector functions: mechanisms for delivery of cytotoxicity and help.
    Annu Rev Cell Biol. 1991;7:479-504 PMID: 1839711
  30. A central role of perforin in cytolysis?
    Annu Rev Immunol. 1991;9:129-57 PMID: 1910674
  31. A monoclonal antibody reactive with a 15-kDa cytoplasmic granule-associated protein defines a subpopulation of CD8+ T lymphocytes.
    J Immunol. 1990 Jan 15;144(2):574-82 PMID: 2104899
  32. Inhibition of lymphocyte mediated cytotoxicity by perforin antisense oligonucleotides.
    EMBO J. 1990 Dec;9(12):3815-9 PMID: 2249651
  33. In vivo expression of perforin by CD8+ lymphocytes during an acute viral infection.
    J Exp Med. 1989 Jun 1;169(6):2159-71 PMID: 2471775
Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1992-05-00
Pages
1025-30
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1886520
Subset
IM
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2