Home LiteratureArticle Details
PMID: 1373507 Published · ppublish English Journal Article

Tyrosine phosphorylation of phospholipase C-gamma 1 induced by cross-linking of the high-affinity or low-affinity Fc receptor for IgG in U937 cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 8 ·1992-04-15 ·Pages 3659-63

Liao F, Shin HS, Rhee SG

Abstract

The human monocytic cell line U937 possesses two classes of the IgG Fc receptor (Fc gamma R), a high-affinity 72-kDa Fc gamma R (Fc gamma RI) and a low-affinity 40-kDa Fc gamma R (Fc gamma RII). Cross-linking of either class of Fc gamma R in U937 cells elicits an increase in the concentration of free intracellular Ca2+. A rapid rise in the concentration of inositol 1,4,5-trisphosphate (Ins-1,4,5-P3) and of several other inositol phosphates derived from Ins-1,4,5-P3 was observed after cross-linking of Fc gamma Rs in U937 cells. This result suggests that Ins-1,4,5-P3, generated by the action of phospholipase C (PLC), acts as a second messenger by which Fc gamma Rs mobilize intracellular Ca2+ in U937 cells. The mechanism by which the cross-linking of Fc gamma Rs triggers activation of PLC was studied. Cross-linking of Fc gamma RI or Fc gamma RII resulted in a rapid and transient phosphorylation of PLC-gamma 1 on tyrosine residues. It has previously been shown that phosphorylation of PLC-gamma 1 on tyrosine residues activates its enzymatic activity in cells. Prior incubation of U937 cells with a protein tyrosine kinase inhibitor, herbimycin A, prevented the tyrosine phosphorylation of PLC-gamma 1 and the hydrolysis of phosphatidylinositol 4,5-bisphosphate induced by the cross-linking of Fc gamma Rs. Thus, Fc gamma RI and Fc gamma RII appear to be functionally coupled to a nonreceptor tyrosine kinase that phosphorylates PLC-gamma 1 after receptor cross-linking, thereby causing activation of PLC-gamma 1.

MeSH Terms
Antigens, Differentiation/metabolism Arsenicals/pharmacology Calcium/metabolism Cell Line Cross-Linking Reagents/pharmacology Humans Immunoglobulin G/metabolism Inositol 1,4,5-Trisphosphate/metabolism Inositol Phosphates/metabolism Kinetics Phosphates/metabolism Phosphatidylinositol 4,5-Diphosphate Phosphatidylinositols/metabolism Phosphorylation Phosphotyrosine Receptors, Fc/metabolism Receptors, IgG Type C Phospholipases/metabolism Tyrosine/analogs & derivatives,analysis
Chemicals
Antigens, Differentiation Arsenicals Cross-Linking Reagents Immunoglobulin G Inositol Phosphates Phosphates Phosphatidylinositol 4,5-Diphosphate Phosphatidylinositols Receptors, Fc Receptors, IgG oxophenylarsine Phosphotyrosine Tyrosine Inositol 1,4,5-Trisphosphate Type C Phospholipases Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Liao F
Department of Molecular Biology and Genetics, Johns Hopkins University, School of Medicine, Baltimore, MD 21205.
Shin H S
Rhee S G
References (35)
35 references, click to expand
  1. Epidermal growth factor stimulates tyrosine phosphorylation of phospholipase C-II independently of receptor internalization and extracellular calcium.
    Proc Natl Acad Sci U S A. 1989 Mar;86(5):1568-72 PMID: 2466293
  2. Inositol 1,3,4,5-tetrakisphosphate and not phosphatidylinositol 3,4-bisphosphate is the probable precursor of inositol 1,3,4-trisphosphate in agonist-stimulated parotid gland.
    Biochem J. 1986 Sep 1;238(2):501-6 PMID: 2432882
  3. Phospholipase C-gamma is a substrate for the PDGF and EGF receptor protein-tyrosine kinases in vivo and in vitro.
    Cell. 1989 Jun 30;57(7):1109-22 PMID: 2472219
  4. Monoclonal antibodies that bind to distinct epitopes on Fc gamma RI are able to trigger receptor function.
    J Immunol. 1989 Sep 1;143(5):1650-5 PMID: 2474608
  5. Human IgG Fc receptor (hFcRII; CD32) exists as multiple isoforms in macrophages, lymphocytes and IgG-transporting placental epithelium.
    EMBO J. 1989 Dec 1;8(12):3657-66 PMID: 2531080
  6. Fc gamma R(CD16) interaction with ligand induces Ca2+ mobilization and phosphoinositide turnover in human natural killer cells. Role of Ca2+ in Fc gamma R(CD16)-induced transcription and expression of lymphokine genes.
    J Exp Med. 1989 Feb 1;169(2):549-67 PMID: 2536067
  7. Acid and base hydrolysis of phosphoproteins bound to immobilon facilitates analysis of phosphoamino acids in gel-fractionated proteins.
    Anal Biochem. 1989 Jan;176(1):22-7 PMID: 2540676
  8. Metabolism of the inositol phosphates produced upon receptor activation.
    Biochem J. 1989 Jun 1;260(2):313-24 PMID: 2548474
  9. Inositol phosphates and cell signalling.
    Nature. 1989 Sep 21;341(6239):197-205 PMID: 2550825
  10. T cell CD3-zeta eta heterodimer expression and coupling to phosphoinositide hydrolysis.
    Science. 1988 Oct 28;242(4878):571-4 PMID: 2845582
  11. Mechanism of human monocyte activation via the 40-kDa Fc receptor for IgG.
    J Immunol. 1988 Dec 15;141(12):4333-43 PMID: 2848894
  12. Isolation and expression of functional high-affinity Fc receptor complementary DNAs.
    Science. 1989 Jan 20;243(4889):378-81 PMID: 2911749
  13. Structure and function of human and murine receptors for IgG.
    Annu Rev Immunol. 1988;6:251-81 PMID: 2968084
  14. Human neutrophil Fc gamma receptor distribution and structure.
    Proc Natl Acad Sci U S A. 1982 May;79(10):3275-9 PMID: 6808506
  15. Inhibition of CD3-linked phospholipase C by phorbol ester and by cAMP is associated with decreased phosphotyrosine and increased phosphoserine contents of PLC-gamma 1.
    J Biol Chem. 1992 Jan 25;267(3):1496-501 PMID: 1370476
  16. Multiple forms of phosphoinositide-specific phospholipase C and different modes of activation.
    Biochem Soc Trans. 1991 Apr;19(2):337-41 PMID: 1653736
  17. Inositol phospholipids-specific phospholipase C: interaction of the gamma 1 isoform with tyrosine kinase.
    Trends Biochem Sci. 1991 Aug;16(8):297-301 PMID: 1659758
  18. Tyrosine residues in bovine phospholipase C-gamma phosphorylated by the epidermal growth factor receptor in vitro.
    J Biol Chem. 1990 Mar 5;265(7):3940-3 PMID: 1689310
  19. Increase of the catalytic activity of phospholipase C-gamma 1 by tyrosine phosphorylation.
    Science. 1990 Nov 30;250(4985):1253-6 PMID: 1700866
  20. The cytoplasmic domain of the T cell receptor zeta chain is sufficient to couple to receptor-associated signal transduction pathways.
    Cell. 1991 Mar 8;64(5):891-901 PMID: 1705867
  21. Activation of the beta 1 isozyme of phospholipase C by alpha subunits of the Gq class of G proteins.
    Nature. 1991 Apr 11;350(6318):516-8 PMID: 1707501
  22. PDGF stimulation of inositol phospholipid hydrolysis requires PLC-gamma 1 phosphorylation on tyrosine residues 783 and 1254.
    Cell. 1991 May 3;65(3):435-41 PMID: 1708307
  23. Functional activation of the T-cell antigen receptor induces tyrosine phosphorylation of phospholipase C-gamma 1.
    Proc Natl Acad Sci U S A. 1991 Jul 1;88(13):5484-8 PMID: 1712101
  24. CD3 stimulation causes phosphorylation of phospholipase C-gamma 1 on serine and tyrosine residues in a human T-cell line.
    Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5453-6 PMID: 1828897
  25. Fc receptors and membrane immunoglobulin.
    Curr Opin Immunol. 1991 Feb;3(1):40-6 PMID: 1828949
  26. Regulation of polyphosphoinositide-specific phospholipase C activity by purified Gq.
    Science. 1991 Feb 15;251(4995):804-7 PMID: 1846707
  27. Cellular immunity to HIV activated by CD4 fused to T cell or Fc receptor polypeptides.
    Cell. 1991 Mar 8;64(5):1037-46 PMID: 1900456
  28. Fc receptors.
    Annu Rev Immunol. 1991;9:457-92 PMID: 1910686
  29. Tyrosine phosphorylation of phospholipase C induced by membrane immunoglobulin in B lymphocytes.
    Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2745-9 PMID: 2011584
  30. IgM antigen receptor complex contains phosphoprotein products of B29 and mb-1 genes.
    Proc Natl Acad Sci U S A. 1991 May 1;88(9):3982-6 PMID: 2023945
  31. Tyrosine phosphorylation provides an early and requisite signal for the activation of natural killer cell cytotoxic function.
    Proc Natl Acad Sci U S A. 1991 Jul 15;88(14):6279-83 PMID: 2068107
  32. Cross-linking of both types of IgG Fc receptors, Fc gamma RI and Fc gamma RII, enhances intracellular free Ca2+ in the monocytic cell line U937.
    Scand J Immunol. 1990 Mar;31(3):315-25 PMID: 2157275
  33. Family of disulphide-linked dimers containing the zeta and eta chains of the T-cell receptor and the gamma chain of Fc receptors.
    Nature. 1990 Sep 13;347(6289):189-91 PMID: 2203969
  34. Inhibition of tyrosine phosphorylation prevents T-cell receptor-mediated signal transduction.
    Proc Natl Acad Sci U S A. 1990 Oct;87(19):7722-6 PMID: 2217205
  35. Activation of human monocytes occurs on cross-linking monocytic antigens to an Fc receptor.
    J Immunol. 1989 Apr 1;142(7):2377-83 PMID: 2466895
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.8.3659
Published
1992-04-15
Pages
3659-63
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48928
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.8.3659 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2