Home LiteratureArticle Details
PMID: 1371961 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The role of the IGF1 receptor in the regulation of cdc2 mRNA levels in fibroblasts.

Experimental cell research ·Vol. 199 ·No. 2 ·1992-04-00 ·Pages 275-8

Surmacz E, Nugent P, Pietrzkowski Z, Baserga R

Abstract

The levels of cdc2 mRNA increase when quiescent cells are stimulated by growth factors. In BALB/c 3T3, both platelet-derived growth factor and insulin-like growth factor 1 (IGF-1) are required to increase cdc2 mRNA levels. In p6 cells, which constitutively overexpress the IGF-1 receptor, IGF-1 is sufficient. The importance of the IGF-1/IGF-1 receptor interaction in regulating the levels of cdc2 mRNA was further confirmed by showing that an antisense oligodeoxynucleotide to the IGF-1 receptor RNA inhibited the IGF-1-mediated increase.

MeSH Terms
3T3 Cells Animals Blotting, Northern CDC2 Protein Kinase/genetics Culture Media, Serum-Free Fibroblasts/metabolism Gene Expression Regulation Humans Insulin-Like Growth Factor I/metabolism Mice Mice, Inbred BALB C Platelet-Derived Growth Factor/metabolism Polymerase Chain Reaction RNA, Messenger/metabolism RNA-Directed DNA Polymerase/metabolism Receptors, Cell Surface/genetics,metabolism,physiology Receptors, Somatomedin Transcription, Genetic Transfection
Chemicals
Culture Media, Serum-Free Platelet-Derived Growth Factor RNA, Messenger Receptors, Cell Surface Receptors, Somatomedin Insulin-Like Growth Factor I CDC2 Protein Kinase RNA-Directed DNA Polymerase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Surmacz E
Jefferson Cancer Institute, Thomas Jefferson University, Jefferson Medical College, Philadelphia, Pennsylvania 19107.
Nugent P
Pietrzkowski Z
Baserga R
Article Info
Journal
Experimental cell research
Abbr.
Exp Cell Res
ISSN
0014-4827
Published
1992-04-00
Pages
275-8
Language
English
Region
United States
NLM ID
0373226
Subset
IM
Grants
NIGMS NIH HHS · GM-42383 · United States
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com