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PMID: 1371510 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Biochemical properties of chimeric skeletal and smooth muscle myosin light chain kinases.

The Journal of biological chemistry ·Vol. 267 ·No. 7 ·1992-03-05 ·Pages 4930-8

Leachman SA, Gallagher PJ, Herring BP, McPhaul MJ, Stull JT

Abstract

The molecular and biochemical properties of myosin light chain kinases from chicken skeletal and smooth muscle were investigated by recombinant DNA techniques. Deletion of the amino-terminal region of either the smooth or skeletal muscle myosin light chain kinase resulted in a decrease in Vmax with no significant change in Km values for light chain substrates. Skeletal/smooth muscle chimeric kinases were inactive when a 65-residue region amino-terminal of the catalytic core was exchanged between the two forms. Changing alanine 494 to glutamic acid within this region in the chicken skeletal muscle myosin light chain kinase increased the Km values for light chains 10-fold. These results are consistent with the hypothesis that the region amino-terminal of the catalytic core in myosin light chain kinases is involved in light chain recognition. A skeletal muscle kinase which contained the smooth muscle calmodulin binding domain remained regulated by Ca2+/calmodulin. Thus, the calmodulin binding domains of smooth and skeletal muscle myosin light chain kinases share structural elements necessary for regulation.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern Blotting, Western Calmodulin/metabolism Chickens DNA/genetics DNA, Recombinant/metabolism Kinetics Molecular Sequence Data Muscle, Smooth/enzymology Muscles/enzymology Myosin-Light-Chain Kinase/genetics,metabolism RNA/genetics Recombinant Fusion Proteins/genetics,metabolism Restriction Mapping Sequence Homology, Nucleic Acid Substrate Specificity Transcription, Genetic
Chemicals
Calmodulin DNA, Recombinant Recombinant Fusion Proteins RNA DNA Myosin-Light-Chain Kinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Leachman S A
Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235.
Gallagher P J
Herring B P
McPhaul M J
Stull J T
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
0021-9258
Published
1992-03-05
Pages
4930-8
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC2836765
Subset
IM
Grants
NIGMS NIH HHS · T32 GM008014 · United States
NHLBI NIH HHS · R01 HL026043 · United States
NHLBI NIH HHS · R01 HL026043-20 · United States
NHLBI NIH HHS · R37 HL026043 · United States
NIGMS NIH HHS · 5T-32 GM08014 · United States
NHLBI NIH HHS · HL26043 · United States
Databases
GENBANK
L01674, L01675, L01676, L01677, M81786, M81787, M83326, M83327, M83328, M83368
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PubMed source PMC Full Text View journal details
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