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PMID: 1370275 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Immunochemical studies and complete amino acid sequence of the streptokinase from Streptococcus pyogenes (group A) M type 12 strain A374.

Infection and immunity ·Vol. 60 ·No. 1 ·1992-01-00 ·Pages 278-83

Ohkuni H, Todome Y, Suzuki H, Mizuse M, Kotani N, Horiuchi K, Shikama N, Tsugita A, Johnston KH

Abstract

The complete amino acid sequence of the streptokinase (SKase) of Streptococcus pyogenes M type 12 strain A374, isolated from a patient with poststreptococcal glomerulonephritis (PSGN), was determined. The epitope domain for the monoclonal antibody N-59, which cross-reacts with SKases of both the PSGN-associated strain and S. equisimilis H46A (a non-PSGN-associated strain), was predicted to be localized in residues 370 to 374. The epitope domain specific for monoclonal antibody RU-1, which reacts only with the PSGN-associated SKase, was localized to residues 164 to 236.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Blotting, Western Chromosome Mapping Electrophoresis, Polyacrylamide Gel Epitopes/genetics Molecular Sequence Data Sequence Homology, Nucleic Acid Streptococcus pyogenes/enzymology Streptokinase/genetics,immunology
Chemicals
Amino Acids Epitopes Streptokinase
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Ohkuni H
Division of Immunology, Nippon Medical School, Kanagawa, Japan.
Todome Y
Suzuki H
Mizuse M
Kotani N
Horiuchi K
Shikama N
Tsugita A
Johnston K H
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.60.1.278-283.1992
Published
1992-01-00
Pages
278-83
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC257533
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.60.1.278-283.1992 PMC Full Text View journal details
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