Influenza A virus transfectants with chimeric hemagglutinins containing epitopes from different subtypes.
Influenza virus transfectants with chimeric hemagglutinins were constructed by using a ribonucleoprotein transfection method. Transfectants W(H1)-H2 and W(H1)-H3 contained A/WSN/33(H1N1) (WSN) hemagglutinins in which the six-amino-acid loop (contained in antigenic site B) was replaced by the corresponding structures of influenza viruses A/Japan/57(H2N2) and A/Hong Kong/8/68(H3N2) (HK), respectively. Serological analysis indicated that the W(H1)-H3 transfectant virus reacted with antibodies against both the WSN and HK viruses in hemagglutination inhibition and plaque neutralization assays. Furthermore, mice immunized with W(H1)-H3 transfectant virus produced antibodies to the WSN and HK viruses. The results demonstrate that influenza virus transfectants can be engineered to express epitopes of different subtypes on their hemagglutinins.
15 references, click to expand
-
The antigenic structure of the influenza virus A/PR/8/34 hemagglutinin (H1 subtype).Cell. 1982 Dec;31(2 Pt 1):417-27 PMID: 6186384
-
An influenza A virus containing influenza B virus 5' and 3' noncoding regions on the neuraminidase gene is attenuated in mice.Proc Natl Acad Sci U S A. 1991 Jun 15;88(12):5177-81 PMID: 2052599
-
Cloning and DNA sequence of double-stranded copies of haemagglutinin genes from H2 and H3 strains elucidates antigenic shift and drift in human influenza virus.Nature. 1980 Sep 25;287(5780):301-6 PMID: 7421990
-
High-efficiency formation of influenza virus transfectants.J Virol. 1991 May;65(5):2711-3 PMID: 2016777
-
Molecular characterization of a new hemagglutinin, subtype H14, of influenza A virus.Virology. 1990 Dec;179(2):759-67 PMID: 2238469
-
Introduction of site-specific mutations into the genome of influenza virus.Proc Natl Acad Sci U S A. 1990 May;87(10):3802-5 PMID: 2339122
-
Antigen chimaeras of poliovirus as potential new vaccines.Nature. 1988 Mar 3;332(6159):81-2 PMID: 2450279
-
An engineered poliovirus chimaera elicits broadly reactive HIV-1 neutralizing antibodies.Nature. 1989 Jun 1;339(6223):385-8, 340 PMID: 2542797
-
Amplification, expression, and packaging of foreign gene by influenza virus.Cell. 1989 Dec 22;59(6):1107-13 PMID: 2598262
-
Vaccines for the Third World.Nature. 1989 Nov 9;342(6246):115-20 PMID: 2812009
-
Structural identification of the antibody-binding sites of Hong Kong influenza haemagglutinin and their involvement in antigenic variation.Nature. 1981 Jan 29;289(5796):373-8 PMID: 6162101
-
Antigenic structure of influenza virus haemagglutinin defined by hybridoma antibodies.Nature. 1981 Apr 23;290(5808):713-7 PMID: 6163993
-
Positive Darwinian evolution in human influenza A viruses.Proc Natl Acad Sci U S A. 1991 May 15;88(10):4270-4 PMID: 1840695
-
Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 A resolution.Nature. 1981 Jan 29;289(5796):366-73 PMID: 7464906
-
Complete sequence analysis shows that the hemagglutinins of the H0 and H2 subtypes of human influenza virus are closely related.Virology. 1981 May;111(1):113-24 PMID: 7233828
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
