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PMID: 1369475 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

DnaK-mediated alterations in human growth hormone protein inclusion bodies.

Bio/technology (Nature Publishing Company) ·Vol. 10 ·No. 3 ·1992-03-00 ·Pages 301-4

Blum P, Velligan M, Lin N, Matin A

Abstract

Protein overproduction in microbes frequently results in protein misfolding and aggregation though the molecular basis for this process is unclear. The HSP70 chaperonin, DnaK, was identified as an important factor controlling heterologous protein aggregation in Escherichia coli. Co-overproduction of DnaK significantly reduced human growth hormone (HGH) protein inclusion body formation and the extent of HGH aggregation.

MeSH Terms

Bacterial Proteins/metabolism Cell Extracts/isolation & purification Cell Fractionation Chaperonins Escherichia coli/metabolism Growth Hormone/chemistry Macromolecular Substances Plasmids/genetics Protein Conformation Proteins/physiology Solubility

Chemicals

Bacterial Proteins Cell Extracts Macromolecular Substances Proteins Growth Hormone Chaperonins

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Blum P

Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305-5402.

Velligan M

Lin N

Matin A

Article Info

Journal

Bio/technology (Nature Publishing Company)

Abbr.

Biotechnology (N Y)

ISSN

0733-222X

DOI

10.1038/nbt0392-301

Published

1992-03-00

Pages

301-4

Language

English

Region

United States

NLM ID

8309273

External Links

PubMed source DOI: 10.1038/nbt0392-301

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