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PMID: 13693271 Published · ppublish English Journal Article

The inactivation of enterovirus infectivity by the sulfhydryl reagent p-chloromercuribenzoate.

The Journal of experimental medicine ·Vol. 113 ·1961-04-01 ·Pages 713-34

CHOPPIN PW, PHILIPSON L

Abstract

The infectivity of several enteroviruses was inactivated by the sulfhydryl reagent p-chloromercuribenzoate (PCMB). The rate of inactivation was dependent on the ionic environment in which the reaction was carried out. Inactivation of infectivity was reversed by the thiol compound, reduced glutathione. Under certain conditions, PCMB prevented the adsorption of some enteroviruses to monolayer cultures of monkey kidney cells. The results suggest that enterovirus sulfhydryl groups are involved in the establishment of infection, and that they play a role in the adsorption of virus to host cells.

Keywords
BENZOATES/pharmacology INDICATORS AND REAGENTS/pharmacology VIRUSES/pharmacology
MeSH Terms
Benzoates/pharmacology Chloromercuribenzoates Enterovirus Indicators and Reagents/pharmacology Sulfhydryl Compounds Sulfhydryl Reagents Viruses/pharmacology
Chemicals
Benzoates Chloromercuribenzoates Indicators and Reagents Sulfhydryl Compounds Sulfhydryl Reagents
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
CHOPPIN P W
PHILIPSON L
References (20)
20 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.113.4.713
Published
1961-04-01
Pages
713-34
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2137380
Subset
OM
External Links
PubMed source DOI: 10.1084/jem.113.4.713 PMC Full Text View journal details
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