Generation of transgenic dairy cattle using 'in vitro' embryo production.

Bio/technology (Nature Publishing Company) ·Vol. 9 ·No. 9 ·1991-09-00 ·Pages 844-7

Krimpenfort P, Rademakers A, Eyestone W, van der Schans A, van den Broek S, Kooiman P, Kootwijk E, Platenburg G, Pieper F, Strijker R

Abstract

We have combined gene transfer, by microinjection, with 'in vitro' embryo production technology, enabling us to carry out non-surgical transfer, to recipient cows, of microinjected embryos that have been cultured from immature oocytes. Using this approach, we have established 21 pregnancies from which 19 calves were born. Southern blot analysis proved that in two cases the microinjected DNA had been integrated in the host genome.

MeSH Terms

Animals Animals, Genetically Modified Blotting, Southern Caseins/genetics Cattle/genetics Cells, Cultured DNA/genetics,isolation & purification Embryo Transfer Embryonic and Fetal Development Female Humans Lactoferrin/genetics Oocytes/physiology Pregnancy Restriction Mapping Transfection

Chemicals

Caseins DNA Lactoferrin

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Krimpenfort P

Department of Embryology, Gene Pharming Europe B.V., Leiden, The Netherlands.

Rademakers A

Eyestone W

van der Schans A

van den Broek S

Kooiman P

Kootwijk E

Platenburg G

Pieper F

Strijker R

Article Info

Journal

Bio/technology (Nature Publishing Company)

Abbr.

Biotechnology (N Y)

ISSN

0733-222X

DOI

10.1038/nbt0991-844

Published

1991-09-00

Pages

844-7

Language

English

Region

United States

NLM ID

8309273

External Links

PubMed source DOI: 10.1038/nbt0991-844

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