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PMID: 13673141 Published · ppublish English Journal Article

Studies on the mechanism of hypersensitivity phenomena. III. The participation of complement in the formation of anaphylatoxin.

The Journal of experimental medicine ·Vol. 110 ·No. 2 ·1959-08-01 ·Pages 311-39

OSLER AG, RANDALL HG, HILL BM, OVARY Z

Abstract

The interaction of fresh rat and guinea pig serum with washed preformed immune aggregates has been studied with respect to the loss in hemolytic potency of the serum, diminution of the C'(3); activity, and appearance of anaphylatoxin. It has been found that the formation of anaphylatoxin, as judged by its effect on capillary permeability and smooth muscle contraction, is coincident with or subsequent to the fixation of all the known C' components. Less anaphylatoxin is formed by aggregates formed with excess antigen than those in equivalence ratio combination. C' fixation, as well as anaphylatoxin production, may be inhibited by chelation of the divalent cations, presumably by interfering with the fixation of C'(1), C'(4), and C'(2). Phlorizin suppresses the utilization of C'(3) in immune hemolysis, C' fixation by antigen-antibody aggregates, and the production of anaphylatoxin. The biological activities associated with the fixation of C' are not manifest unless C'(3) participates in this process at 37 degrees C. It is concluded that the formation of anaphylatoxin may be regarded as a product of C' fixation.

Keywords
ALLERGY COMPLEMENT
MeSH Terms
Anaphylatoxins Animals Complement System Proteins Guinea Pigs Hemolysis Hypersensitivity Immune System Diseases Rats
Chemicals
Anaphylatoxins Complement System Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
OSLER A G
RANDALL H G
HILL B M
OVARY Z
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42 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.110.2.311
Published
1959-08-01
Pages
311-39
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2136993
Subset
OM
External Links
PubMed source DOI: 10.1084/jem.110.2.311 PMC Full Text View journal details
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