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PMID: 1360475 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Use of randomly amplified polymorphic DNA markers to distinguish isolates of Aspergillus fumigatus.

Journal of clinical microbiology ·Vol. 30 ·No. 11 ·1992-11-00 ·Pages 2991-3

Aufauvre-Brown A, Cohen J, Holden DW

Abstract

Forty-four oligonucleotide decamers were tested for their abilities to generate randomly amplified polymorphic DNA (RAPD) markers from genomic DNAs of three different isolates of Aspergillus fumigatus. Seven primers generated RAPDs that allowed the three isolates to be differentiated; one of the primers also yielded a unique RAPD pattern in each of an additional six fungal isolates, demonstrating the utility of this technique for distinguishing between A. fumigatus isolates.

MeSH Terms
Aspergillosis/microbiology Aspergillus fumigatus/classification,genetics,isolation & purification Base Sequence DNA, Fungal/genetics Gene Amplification Genetic Markers Humans Molecular Sequence Data Polymorphism, Restriction Fragment Length
Chemicals
DNA, Fungal Genetic Markers
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Aufauvre-Brown A
Department of Infectious Diseases and Bacteriology, Royal Postgraduate Medical School, Hammersmith Hospital, London, United Kingdom.
Cohen J
Holden D W
References (12)
12 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.30.11.2991-2993.1992
Published
1992-11-00
Pages
2991-3
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC270566
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.30.11.2991-2993.1992 PMC Full Text View journal details
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