Intracellular distribution of 5' nucleotidase in rat liver.

The Journal of biophysical and biochemical cytology ·Vol. 4 ·No. 4 ·1958-07-25 ·Pages 373-6

DE LAMIRANDE G, ALLARD C, CANTERO A

Abstract

The intracellular distribution of 5' nucleotidase was investigated in rat liver by biochemical analysis of cell fractions obtained by differential centrifugation. The enzymatic activity was measured by determination of the inorganic phosphorus liberated from 5' nucleotides. The 5' nucleotidase activity was mainly found in the nuclear and microsomal fractions. An attempt to extract the enzyme from these fractions with Mg(++) ion solutions was unsuccessful. It is concluded that 5' nucleotidase is actually present in the nuclear and microsomal fractions of rat liver cells.

Keywords

LIVER/metabolism PHOSPHATASES

MeSH Terms

5'-Nucleotidase Acid Phosphatase Animals Cytoplasm Liver/metabolism Phosphoric Monoester Hydrolases Protein Tyrosine Phosphatases Rats

Chemicals

Acid Phosphatase Phosphoric Monoester Hydrolases prostatic acid phosphatase Protein Tyrosine Phosphatases 5'-Nucleotidase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

DE LAMIRANDE G

ALLARD C

CANTERO A

References (7)

7 references, click to expand

Influence of cations on the intracellular distribution of rat liver arginase.
J Biol Chem. 1956 Nov;223(1):469-78 PMID: 13376616
Further studies on the histochemical specificity of phosphatases.
Proc Soc Exp Biol Med. 1949 Nov;72(2):449, illust PMID: 15398337
Histochemical studies of 5 nucleotidase. I. Method and specificity.
Lab Invest. 1952;1(4):480-7 PMID: 13023996
Histochemical Demonstration of 5-Nucleotidase Activity in Cell Nuclei.
Science. 1952 Jun 13;115(2998):652-3 PMID: 17844314
Histochemical studies of 5-nucleotidase. II. 5-Nucleotidase in glomerular obsolescence.
Lab Invest. 1953 Jan-Feb;2(1):76-83; contd PMID: 13036025
Alkaline phosphatase substrate specificities in cultured normal and malignant cells of mouse, rat, and fowl.
Cancer Res. 1951 Mar;11(3):174-9 PMID: 14821957
Mitochondrial population in mammalian cells. I. Description of a counting technic and preliminary results on rat liver in different physiological and pathological conditions.
Cancer Res. 1952 Jun;12(6):407-12 PMID: 14936004

Article Info

Journal

The Journal of biophysical and biochemical cytology

Abbr.

J Biophys Biochem Cytol

ISSN

0095-9901

DOI

10.1083/jcb.4.4.373

Published

1958-07-25

Pages

373-6

Language

English

Region

United States

NLM ID

17840020R

PMCID

PMC2224501

Subset

External Links

PubMed source DOI: 10.1083/jcb.4.4.373 PMC Full Text

Analysis Services

Intracellular distribution of 5' nucleotidase in rat liver.

Contact

WeChat Official Account