Detection of non-precipitating antibodies in sera of individuals allergic to ragweed pollen by an in vitro method.

The Journal of experimental medicine ·Vol. 108 ·No. 1 ·1958-07-01 ·Pages 37-51

GORDON J, ROSE B, SEHON AH

Abstract

A hemagglutination test capable of demonstrating antibodies in sera of ragweed-sensitive individuals has been described in detail. This test involves coupling of the antigen to rabbit erythrocytes via stable azo bonds. The antigen-coated cells are then suspended in the serum which is suspected to contain the homologous antibodies. The test has been shown to be specific and highly sensitive. Precipitating antibodies to ragweed pollen extract produced in experimental animals can be demonstrated in dilutions as high as 10(5) to 10(7), while antibodies to ragweed in allergic sera are detected only in dilutions of the order of 10(3). Positive results were obtained with all sera from treated or non-treated allergic individuals containing skin-sensitizing and/or blocking antibodies. Absorption of these sera with antigen-coated erythrocytes yielded a supernatant devoid of either skin-sensitizing, blocking, or hemagglutinating capacity. It is concluded that the hemagglutinating factor has the properties of a divalent antibody.

Keywords

ANTIBODIES RAGWEED

MeSH Terms

Allergens Ambrosia Antibodies Antibodies, Blocking Antigens Antigens, Plant Humans Immunoglobulin E In Vitro Techniques Oxidation-Reduction Plant Extracts Pollen Rhinitis, Allergic, Seasonal

Chemicals

Allergens Antibodies Antibodies, Blocking Antigens Antigens, Plant Plant Extracts ragweed pollen Immunoglobulin E

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

GORDON J

ROSE B

SEHON A H

References (18)

18 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.108.1.37

Published

1958-07-01

Pages

37-51

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2136885

Subset

External Links

PubMed source DOI: 10.1084/jem.108.1.37 PMC Full Text View journal details

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Detection of non-precipitating antibodies in sera of individuals allergic to ragweed pollen by an in vitro method.

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