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PMID: 1347950 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genomic analysis using a yeast artificial chromosome library with mouse DNA inserts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 6 ·1992-03-15 ·Pages 2456-60

Rossi JM, Burke DT, Leung JC, Koos DS, Chen H, Tilghman SM

Abstract

A yeast artificial chromosome library with mouse genomic DNA inserts has been constructed. The library encompasses a 2.5-fold coverage of the mouse genome, with an average insert size of 250 kilobases. The screening strategy uses the polymerase chain reaction on pooled DNAs prepared from individually stored clones. The usefulness of the library for chromosome walking was illustrated by constructing a 600-kilobase-long contig of DNA surrounding Hba-ps4, a DNA marker that is tightly linked to the fused (Fu) locus on chromosome 17.

Related Genes
Hba-ps4
MeSH Terms
Animals Base Sequence Chromosome Mapping Chromosomes, Fungal DNA/genetics,isolation & purification Exons Female Gene Library Genetic Markers Genome Mice Mice, Inbred C57BL Molecular Sequence Data Oligodeoxyribonucleotides Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Saccharomyces cerevisiae/genetics Spleen/physiology
Chemicals
Genetic Markers Oligodeoxyribonucleotides DNA
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Rossi J M
Howard Hughes Medical Institute, Princeton University, NJ 08544.
Burke D T
Leung J C
Koos D S
Chen H
Tilghman S M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.6.2456
Published
1992-03-15
Pages
2456-60
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC48677
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.89.6.2456 PMC Full Text View journal details
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