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PMID: 13475389 Published · ppublish English Journal Article

Electron microscopy of HeLa cells after the ingestion of colloidal gold.

The Journal of biophysical and biochemical cytology ·Vol. 3 ·No. 5 ·1957-09-25 ·Pages 749-56

HARFORD CG, HAMLIN A, PARKER E

Abstract

Tissue cultures of HeLa cells were grown in media containing colloidal gold, and after various intervals, the cells were fixed, embedded, and sectioned for electron microscopy. Uncoated grids with small holes were used in many of the experiments. Intracellular particles of gold were identified in areas surrounded by single membranes, in moderately dense granules, in globoid bodies, and in the cytoplasmic matrix. Gold particles were not found in typical mitochondria, Golgi complex, ergastoplasm (granular forms of endoplasmic reticulum), or nuclei. The phenomenon of pinocytosis was considered to be the most likely means by which the gold particles were ingested, and the locations of gold particles appeared to have significance concerning theories that membranous organelles of the cytoplasm may be derived from the cell membrane.

Keywords
GOLD/radioactive MICROSCOPY ELECTRON TISSUE CULTURE
MeSH Terms
Animals Cell Nucleus Cytoplasmic Granules Electrons Endoplasmic Reticulum Gold Gold Colloid Gold Radioisotopes Golgi Apparatus HeLa Cells Humans Microscopy Microscopy, Electron Mitochondria Organelles Pinocytosis Research Design Tissue Culture Techniques
Chemicals
Gold Colloid Gold Radioisotopes Gold
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
HARFORD C G
HAMLIN A
PARKER E
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34 references, click to expand
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Article Info
Journal
The Journal of biophysical and biochemical cytology
Abbr.
J Biophys Biochem Cytol
ISSN
0095-9901
DOI
10.1083/jcb.3.5.749
Published
1957-09-25
Pages
749-56
Language
English
Region
United States
NLM ID
17840020R
PMCID
PMC2224119
Subset
OM
External Links
PubMed source DOI: 10.1083/jcb.3.5.749 PMC Full Text
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