Characterization of EntF as a serine-activating enzyme.
EntF is the enzyme responsible for serine activation during the biosynthesis of enterobactin (a cyclic trimer of N-dihydroxybenzoyl serine) in Escherichia coli. EntF has been overexpressed and purified to > 90% homogeneity. The enzyme has been shown to complement the entF- MK1 strain in the synthesis of 2,3-dihydroxybenzoyl serine derivatives and exhibits L-serine-dependent ATP[32P] pyrophosphate exchange activity with a Km for serine of 260 mM and a turnover number of 760 min-1. Release of PPi during incubation of EntF with serine and ATP was observed, but with a low turnover number of 1.0 min-1. These results suggested the presence of an enzyme-bound intermediate, which has been shown by gel filtration analysis to be (L-serine)adenylate.
16 references, click to expand
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Biosynthesis of the Escherichia coli siderophore enterobactin: sequence of the entF gene, expression and purification of EntF, and analysis of covalent phosphopantetheine.Biochemistry. 1991 Mar 19;30(11):2916-27 PMID: 1826089
-
Enniatin synthetase, a novel type of multifunctional enzyme catalyzing depsipeptide synthesis in Fusarium oxysporum.Biochemistry. 1982 Jan 5;21(1):43-8 PMID: 6895851
-
Overexpression, purification, and characterization of isochorismate synthase (EntC), the first enzyme involved in the biosynthesis of enterobactin from chorismate.Biochemistry. 1990 Feb 13;29(6):1417-25 PMID: 2139795
-
Subcloning of the enterobactin biosynthetic gene entB: expression, purification, characterization, and substrate specificity of isochorismatase.Biochemistry. 1990 Feb 13;29(6):1425-35 PMID: 2139796
-
Cyclosporin synthetase. The most complex peptide synthesizing multienzyme polypeptide so far described.J Biol Chem. 1990 Jul 5;265(19):11355-60 PMID: 2358465
-
Nucleotide sequence of a cluster of Escherichia coli enterobactin biosynthesis genes: identification of entA and purification of its product 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase.J Bacteriol. 1989 Feb;171(2):791-8 PMID: 2521622
-
Subcloning, expression, and purification of the enterobactin biosynthetic enzyme 2,3-dihydroxybenzoate-AMP ligase: demonstration of enzyme-bound (2,3-dihydroxybenzoyl)adenylate product.Biochemistry. 1989 Aug 22;28(17):6827-35 PMID: 2531000
-
Molecular characterization of the Escherichia coli enterobactin cistron entF and coupled expression of entF and the fes gene.J Bacteriol. 1987 Sep;169(9):4154-62 PMID: 3040679
-
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.J Mol Biol. 1986 May 5;189(1):113-30 PMID: 3537305
-
Altered molecular form of acyl carrier protein associated with beta-ketoacyl-acyl carrier protein synthase II (fabF) mutants.J Bacteriol. 1987 Apr;169(4):1469-73 PMID: 3549687
-
Human lipoprotein lipase complementary DNA sequence.Science. 1987 Mar 27;235(4796):1638-41 PMID: 3823907
-
The 3'-terminal sequence of Escherichia coli 16S ribosomal RNA: complementarity to nonsense triplets and ribosome binding sites.Proc Natl Acad Sci U S A. 1974 Apr;71(4):1342-6 PMID: 4598299
-
Regulation of coenzyme A biosynthesis.J Bacteriol. 1981 Dec;148(3):926-32 PMID: 6796563
-
Studies on the enzymatic synthesis of the cyclic trimer of 2,3-dihydroxy-N-benzoyl-L-serine in Escherichia coli.Biochemistry. 1972 Apr 25;11(9):1708-15 PMID: 4337557
-
Acyl carrier protein. 8. Studies of acyl carrier protein and coenzyme A in Escherichia coli pantothenate or betaalanine auxotrophs.J Biol Chem. 1966 Nov 25;241(22):5201-4 PMID: 5333155
-
Structure of human pancreatic lipase.Nature. 1990 Feb 22;343(6260):771-4 PMID: 2106079
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