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PMID: 1333604 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Evidence for a base-pairing interaction between U6 small nuclear RNA and 5' splice site during the splicing reaction in yeast.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 23 ·1992-12-01 ·Pages 11269-73

Sawa H, Abelson J

Abstract

U6 small nuclear RNA (snRNA) is an essential factor in mRNA splicing. On the basis of the high conservation of its sequence, it has been proposed that U6 snRNA may function catalytically during the splicing reaction. If this is the case, it is likely that U6 snRNA interacts with the splice sites in the spliceosome to catalyze the reaction. We have used UV crosslinking to analyze the interactions of U6 snRNA with the splicing substrates during the yeast splicing reaction. Crosslinked products in which the central region of U6 snRNA was joined to the 5' splice site region of mRNA precursor and lariat intermediate were identified. The crosslinking sites were precisely located in one of these products. The results suggest a possible base-pairing interaction between U6 snRNA and the 5' splice site of the mRNA precursor.

MeSH Terms
Base Sequence Consensus Sequence Hydrogen Bonding Molecular Sequence Data RNA Splicing RNA, Fungal/metabolism RNA, Small Nuclear/metabolism Ribonuclease H/pharmacology Saccharomyces cerevisiae Spliceosomes/metabolism Ultraviolet Rays
Chemicals
RNA, Fungal RNA, Small Nuclear Ribonuclease H
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sawa H
California Institute of Technology, Division of Biology, Pasadena 91125.
Abelson J
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49 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.23.11269
Published
1992-12-01
Pages
11269-73
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50531
Subset
IM
Grants
NIGMS NIH HHS · GM 32637 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.23.11269 PMC Full Text View journal details
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