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PMID: 133352 Published · ppublish English Journal Article

Calcium control of actin-activated myosin adenosine triphosphatase from Dictyostelium discoideum.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 7 ·1976-07-00 ·Pages 2321-5

Mockrin SC, Spudich JA

Abstract

A protein fraction from the cellular slime mold Dictyostelium discoideum confers Ca2+-sensitivity on the activation of purified myosin adenosinetriphosphatase (ATP phosphohydrolase, EC 3.6.1.3) from Dictyostelium by purified Dictyostelium actin. That is, the fraction inhibits the actomyosin adenosine triphosphatase activity in the absence of Ca+ but not in the presence of Ca2+. This Ca2+-sensitizing factor affects only the actin-activated myosin adenosine triphosphatase and not the enzyme activity of myosin alone. The Ca2+-sensitivity is conserved when muscle actin replaces Dictyostelium actin, but is lost when muscle myosin replaces Dictyostelium myosin. The factor appears to be a protein since it is nondialyzable, is heat labile, and can be precipitated with ammonium sulfate. The factor can be purified 70-fold on an actin-affinity column.

MeSH Terms
Actins/metabolism Adenosine Triphosphatases/metabolism Calcium/pharmacology Dictyostelium/metabolism Fungal Proteins/isolation & purification,metabolism Myosins/metabolism Myxomycetes/metabolism Species Specificity
Chemicals
Actins Fungal Proteins Adenosine Triphosphatases Myosins Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mockrin S C
Spudich J A
References (30)
30 references, click to expand
  1. Supramolecular forms of actin from amoebae of Dictyostelium discoideum.
    J Biol Chem. 1975 Sep 25;250(18):7485-91 PMID: 1172503
  2. Ca2+-sensitivity of actomyosin ATPase purified from Physarum polycephalum.
    J Biochem. 1975 Jun;77(6):1127-34 PMID: 131790
  3. Physarum tropomyosin-troponin complex. Isolation and properties.
    J Biochem. 1975 Sep;78(3):583-8 PMID: 131791
  4. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  5. Determination of protein: a modification of the Lowry method that gives a linear photometric response.
    Anal Biochem. 1972 Aug;48(2):422-7 PMID: 4115981
  6. Resolution of bacterial proteins by polyacrylamide gel electrophoresis on slabs. Membrane, soluble, and periplasmic fractions.
    J Biol Chem. 1974 Jan 25;249(2):634-44 PMID: 4129205
  7. Actin is the naturally occurring inhibitor of deoxyribonuclease I.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):4742-6 PMID: 4140510
  8. Role of myosin light chains in calcium regulation.
    Nature. 1974 Jun 14;249(458):631-4 PMID: 4209266
  9. Protoplasmic movements within cells.
    Physiol Rev. 1969 Oct;49(4):793-862 PMID: 4241846
  10. The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.
    J Biol Chem. 1971 Aug 10;246(15):4866-71 PMID: 4254541
  11. A contractile protein possessing Ca 2+ sensitivity (natural actomyosin) from leucocytes. Its extraction and some of its properties.
    Biochim Biophys Acta. 1972 Feb 28;256(2):565-76 PMID: 4259563
  12. The light chains of scallop myosin as regulatory subunits.
    J Mol Biol. 1973 Feb 25;74(2):179-203 PMID: 4266076
  13. Inhibition of Mg ++ ATPase activity of actin-activated Acanthamoeba myosin by muscle troponin-tropomyosin: implications for the mechanism of control of amoeba motility and muscle contraction.
    Biochem Biophys Res Commun. 1973 Apr 2;51(3):693-8 PMID: 4267405
  14. Tropomyosin in brain and growing neurones.
    Nat New Biol. 1973 Oct 10;245(145):182-6 PMID: 4270271
  15. Actin-linked regulation of the human platelet contractile system.
    FEBS Lett. 1973 Aug 15;34(2):315-7 PMID: 4270615
  16. Actin and myosin and cell movement.
    CRC Crit Rev Biochem. 1974 Jan;2(1):1-65 PMID: 4273099
  17. Biochemical and structural studies of actomyosin-like proteins from non-muscle cells. Isolation and characterization of myosin from amoebae of Dictyostelium discoideum.
    J Mol Biol. 1974 Jun 25;86(2):209-22 PMID: 4278009
  18. Biochemical and structural studies of actomyosin-like proteins from non-muscle cells. II. Purification, properties, and membrane association of actin from amoebae of Dictyostelium discoideum.
    J Biol Chem. 1974 Sep 25;249(18):6013-20 PMID: 4278010
  19. Actin mediated calcium dependency of actomyosin in a myxomycete.
    Biochem Biophys Res Commun. 1974 Sep 23;60(2):656-64 PMID: 4278827
  20. 3'5' AMP and Ca 2+ in slime mold aggregation.
    Exp Cell Res. 1971 Jul;67(1):156-60 PMID: 4328063
  21. Molecular control mechanisms in muscle contraction.
    Physiol Rev. 1973 Jul;53(3):612-73 PMID: 4577547
  22. The contractile basis of amoeboid movement. I. The chemical control of motility in isolated cytoplasm.
    J Cell Biol. 1973 Nov;59(2 Pt 1):378-94 PMID: 4805006
  23. Extraction of native tropomyosin-like substances from myxomycete plasmodium and the cross reaction between plasmodium F-actin and muscle native tropomyosin.
    Biochim Biophys Acta. 1972 Feb 29;257(2):445-51 PMID: 5063250
  24. A tropomyosin-like protein from human platelets.
    J Mol Biol. 1972 Jul 21;68(2):383-7 PMID: 5069793
  25. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  26. Specific effect of Ca2+ on movement of plasmodial fragment obtained by caffeine treatment.
    Exp Cell Res. 1970 Jul;61(1):199-203 PMID: 5464472
  27. Microfilaments in cellular and developmental processes.
    Science. 1971 Jan 15;171(3967):135-43 PMID: 5538822
  28. Binding of glycolytic enzymes to structure proteins of the muscle.
    Eur J Biochem. 1968 Nov;6(2):163-71 PMID: 5725503
  29. On the molecular weight of myosin. II.
    Biochemistry. 1966 Feb;5(2):515-21 PMID: 5942951
  30. Chromatography of myosin on diethylaminoethyl-Sephadex A-50.
    Biochemistry. 1967 Feb;6(2):528-40 PMID: 6047636
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.7.2321
Published
1976-07-00
Pages
2321-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430547
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.7.2321 PMC Full Text View journal details
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