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PMID: 13319657 Published · ppublish English Journal Article

Oxidative enzyme systems of the honey bee, Apis mellifera L.

The Journal of general physiology ·Vol. 39 ·No. 5 ·1956-05-20 ·Pages 705-13

HOSKINS DD, CHELDELIN VH, NEWBURGH RW

Abstract

1. Oxidative dissimilation has been studied in enzymes from the honey bee. Using mitochondria isolated from the thoraces, complete oxidation of most of the TCA cycle members has been shown. 2. The presence of the acetate-activating enzyme, citrate-condensing enzyme, isocitric dehydrogenase, alpha-ketoglutarate dehydrogenase, glucose-6-phosphate, and 6-phosphogluconic dehydrogenase has been demonstrated and the cofactor requirements established. 3. The oxidation of isocitric acid has been shown to be either non-specific for the D- or L-isomer, or the presence of a racemase is indicated. 4. The presence of the pentose cycle is indicated in the soluble portion of the thoracic homogenate.

Keywords
BEES/metabolism CARBOHYDRATES/metabolism
MeSH Terms
Animals Bees/metabolism Carbohydrate Metabolism Carbohydrates
Chemicals
Carbohydrates
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
HOSKINS D D
CHELDELIN V H
NEWBURGH R W
References (7)
7 references, click to expand
  1. Malonate inhibition of oxidations in the Krebs tricarboxylic acid cycle.
    J Biol Chem. 1949 Mar;178(1):241-50 PMID: 18112108
  2. Investigations on the mitochondria of the house fly, Musca domestica L. I. Adenosinetriphosphatases.
    J Gen Physiol. 1953 Jan;36(3):371-87 PMID: 13022933
  3. Transfer of C By Lupine Mitochondria Through Reactions of the Tricarboxylic Acid Cycle.
    Proc Natl Acad Sci U S A. 1953 Aug;39(8):754-9 PMID: 16589330
  4. Mitochondria in the flight muscles of insects. II. Effects of the medium on the size form, and organization of isolated sarcosomes.
    J Gen Physiol. 1953 Sep;37(1):71-90 PMID: 13084893
  5. The pathway of oxidation of acetate in baker's yeast.
    Biochem J. 1952 Aug;51(5):614-28 PMID: 13018136
  6. The synthesis of glucose-6-phosphate and 6-phosphogluconate.
    J Biol Chem. 1951 Sep;192(1):175-80 PMID: 14917662
  7. The occurrence of a direct oxidative pathway of carbohydrate metabolism in the fly Musca domestica L.
    Biochim Biophys Acta. 1955 Jun;17(2):294-6 PMID: 13239684
Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
0022-1295
Published
1956-05-20
Pages
705-13
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC2147567
Subset
OM
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