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PMID: 1329095 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A 5' to 3' exonuclease functionally interacts with calf DNA polymerase epsilon.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 89 ·No. 20 ·1992-10-15 ·Pages 9377-81

Siegal G, Turchi JJ, Myers TW, Bambara RA

Abstract

Analysis of fractions containing purified DNA polymerase epsilon from calf thymus has revealed the presence of a 5' to 3' exonuclease activity that is specific for a single strand of duplex DNA. This activity is capable of degrading a 3'-labeled oligonucleotide hybridized to M13mp18 DNA. When a second oligonucleotide primer is annealed 3 bases upstream, degradation of the downstream primer is strictly dependent on DNA synthesis from the upstream primer. Replacement of the downstream primer by an oligoribonucleotide of identical sequence results in a similar pattern of exonucleolytic activity. The activity has been highly purified and found to cosediment in glycerol gradients with a peptide of 56 kDa as judged by SDS/PAGE analysis. Effects of calf DNA polymerase alpha and delta on exonuclease activity are also observed but with differences in the pattern of products.

MeSH Terms
Animals Base Sequence Cattle DNA/metabolism DNA Polymerase II/metabolism DNA Polymerase III DNA Replication DNA-Directed DNA Polymerase/metabolism Exonucleases/metabolism Macromolecular Substances Molecular Sequence Data Nucleic Acid Synthesis Inhibitors Oligodeoxyribonucleotides/chemistry Substrate Specificity Thymus Gland/enzymology
Chemicals
Macromolecular Substances Nucleic Acid Synthesis Inhibitors Oligodeoxyribonucleotides DNA DNA Polymerase II DNA Polymerase III DNA-Directed DNA Polymerase Exonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Siegal G
Department of Biochemistry, University of Rochester School of Medicine and Dentistry, NY 14642.
Turchi J J
Myers T W
Bambara R A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.89.20.9377
Published
1992-10-15
Pages
9377-81
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC50134
Subset
IM
Grants
NCI NIH HHS · CA 46148 · United States
NIGMS NIH HHS · GM 24441 · United States
NCI NIH HHS · T32-CA09363 · United States
External Links
PubMed source DOI: 10.1073/pnas.89.20.9377 PMC Full Text View journal details
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