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PMID: 1322884 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

NAD(P)H-dependent chromium (VI) reductase of Pseudomonas ambigua G-1: a Cr(V) intermediate is formed during the reduction of Cr(VI) to Cr(III).

Journal of bacteriology ·Vol. 174 ·No. 16 ·1992-08-00 ·Pages 5340-5

Suzuki T, Miyata N, Horitsu H, Kawai K, Takamizawa K, Tai Y, Okazaki M

Abstract

An NAD(P)H-dependent Cr(VI) reductase (molecular weight = 65,000) was purified from a Cr(VI)-resistant bacterium, Pseudomonas ambigua G-1. Stoichiometric analysis of the enzymatic reaction showed that the enzyme catalyzed the reduction of 1 mol of Cr(VI) to Cr(III) while consuming 3 mol of NADH as an electron donor. Chromium(VI) was reduced to Cr(V) by one equivalent NADH molecule in the absence of the enzyme. Electron spin resonance analysis showed that Cr(V) species (g = 1.979) was formed during the enzymatic reduction. The amount of Cr(V) species formed was about 10 times larger than that of the nonezymatic reduction. These findings show that the Cr(VI) reductase reduced Cr(VI) to Cr(III) with at least two reaction steps via Cr(V) as an intermediate.

MeSH Terms
Chromatography, Liquid Chromium/chemistry Electron Spin Resonance Spectroscopy NADP/metabolism Oxidation-Reduction Oxidoreductases/metabolism Pseudomonas/enzymology
Chemicals
Chromium NADP Oxidoreductases chromium(VI) reductase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Suzuki T
Department of Biotechnology, Faculty of Agriculture, Gifu University, Japan.
Miyata N
Horitsu H
Kawai K
Takamizawa K
Tai Y
Okazaki M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.174.16.5340-5345.1992
Published
1992-08-00
Pages
5340-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206371
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.174.16.5340-5345.1992 PMC Full Text View journal details
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