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PMID: 1320627 Published · ppublish English Journal Article

A serotype 10 human rotavirus.

Journal of clinical microbiology ·Vol. 30 ·No. 6 ·1992-06-00 ·Pages 1432-5

Beards G, Xu L, Ballard A, Desselberger U, McCrae MA

Abstract

Rotaviruses with genome rearrangements isolated from a chronically infected immunodeficient child (F. Hundley, M. McIntyre, B. Clark, G. Beards, D. Wood, I. Chrystie, and U. Desselberger, J. Virol 61:3365-3372, 1987) are the first recognized human isolates of serotype 10. This was shown by both a direct enzyme-linked immunosorbent assay and virus neutralization assays using serotype specific monoclonal antibodies. The serotype was confirmed by sequence analysis of the gene encoding VP7, which revealed a 96% amino acid homology to the bovine serotype 10 isolate B223.

MeSH Terms
Amino Acid Sequence Antibodies, Monoclonal Base Sequence Enzyme-Linked Immunosorbent Assay Humans Molecular Sequence Data Neutralization Tests Rotavirus/classification,genetics Sequence Homology, Nucleic Acid Serotyping/methods
Chemicals
Antibodies, Monoclonal
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Beards G
Regional Virus Laboratory, East Birmingham Hospital, United Kingdom.
Xu L
Ballard A
Desselberger U
McCrae M A
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27 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
Published
1992-06-00
Pages
1432-5
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC265305
Subset
IM
Databases
GENBANK
X63156
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