Abstract
Rotaviruses with genome rearrangements isolated from a chronically infected immunodeficient child (F. Hundley, M. McIntyre, B. Clark, G. Beards, D. Wood, I. Chrystie, and U. Desselberger, J. Virol 61:3365-3372, 1987) are the first recognized human isolates of serotype 10. This was shown by both a direct enzyme-linked immunosorbent assay and virus neutralization assays using serotype specific monoclonal antibodies. The serotype was confirmed by sequence analysis of the gene encoding VP7, which revealed a 96% amino acid homology to the bovine serotype 10 isolate B223.
MeSH Terms
Amino Acid Sequence
Antibodies, Monoclonal
Base Sequence
Enzyme-Linked Immunosorbent Assay
Humans
Molecular Sequence Data
Neutralization Tests
Rotavirus/classification,genetics
Sequence Homology, Nucleic Acid
Serotyping/methods
Chemicals
Antibodies, Monoclonal
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Beards G
Regional Virus Laboratory, East Birmingham Hospital, United Kingdom.
Xu L
Ballard A
Desselberger U
McCrae M A
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