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PMID: 1316182 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cryo-EM of the native structure of the calcium release channel/ryanodine receptor from sarcoplasmic reticulum.

Biophysical journal ·Vol. 61 ·No. 4 ·1992-04-00 ·Pages 936-40

Radermacher M, Wagenknecht T, Grassucci R, Frank J, Inui M, Chadwick C, Fleischer S

Abstract

The native structure of the calcium release channel (ryanodine receptor) from rabbit skeletal muscle has been analyzed in two dimensions from electron micrographs of frozen hydrated specimens. Within a resolution of 3.0 nm there is excellent agreement between the structure as seen in vitreous water and in negative stained specimens. Features seen in the three-dimensional reconstruction of the negatively stained channel can be identified in the projection of the unstained receptor.

MeSH Terms
Animals Biophysical Phenomena Biophysics Calcium Channels/ultrastructure Freezing Microscopy, Electron Muscle Proteins/ultrastructure Protein Conformation Rabbits Receptors, Cholinergic/ultrastructure Ryanodine Receptor Calcium Release Channel Sarcoplasmic Reticulum/ultrastructure
Chemicals
Calcium Channels Muscle Proteins Receptors, Cholinergic Ryanodine Receptor Calcium Release Channel
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Radermacher M
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
Wagenknecht T
Grassucci R
Frank J
Inui M
Chadwick C
Fleischer S
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(92)81900-8
Published
1992-04-00
Pages
936-40
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1260352
Subset
IM
Grants
NIGMS NIH HHS · 1R01 GM29169 · United States
NCRR NIH HHS · 1S10 RR03998 · United States
NIDDK NIH HHS · DK-14632 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(92)81900-8 PMC Full Text View journal details
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