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PMID: 1311338 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Development and clinical significance of a diagnostic assay based on the polymerase chain reaction for detection of human cytomegalovirus DNA in blood samples from immunocompromised patients.

Journal of clinical microbiology ·Vol. 30 ·No. 2 ·1992-02-00 ·Pages 527-30

Zipeto D, Revello MG, Silini E, Parea M, Percivalle E, Zavattoni M, Milanesi G, Gerna G

Abstract

The presence of human cytomegalovirus (HCMV) DNA in blood was investigated by the polymerase chain reaction (PCR) in 293 blood samples from 86 immunocompromised patients. Of the 86 patients, 23 underwent clinical and virologic follow-up for HCMV infection. In parallel, blood samples were examined for viremia and antigenemia. Concordant results between PCR and assays for viremia and antigenemia were obtained on 124 positive and 110 negative samples, with an overall concordance of 79.8%, while 59 samples (most from patients with HCMV infection) were positive by PCR alone. PCR is a new powerful tool for detection of HCMV infections in blood samples from immunocompromised patients. However, its clinical significance appears to be restricted to the indication of a risk of reactivation of HCMV infection.

MeSH Terms
Antigens, Viral/blood Cytomegalovirus/genetics,isolation & purification Cytomegalovirus Infections/diagnosis DNA, Viral/blood Evaluation Studies as Topic Humans Immunocompromised Host Polymerase Chain Reaction/methods Viremia/diagnosis Virology/methods
Chemicals
Antigens, Viral DNA, Viral
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Zipeto D
Istituto di Genetica, Biochimica ed Evoluzionistica del Consiglio Nazionale delle Ricerche, Pavia, Italy.
Revello M G
Silini E
Parea M
Percivalle E
Zavattoni M
Milanesi G
Gerna G
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.30.2.527-530.1992
Published
1992-02-00
Pages
527-30
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC265096
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.30.2.527-530.1992 PMC Full Text View journal details
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