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PMID: 1309893 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequential phosphorylation of the phosphoprotein of vesicular stomatitis virus by cellular and viral protein kinases is essential for transcription activation.

Journal of virology ·Vol. 66 ·No. 2 ·1992-02-00 ·Pages 1109-18

Barik S, Banerjee AK

Abstract

The phosphoprotein (P) and the large protein (L) constitute the RNA-dependent RNA polymerase of vesicular stomatitis virus (VSV). We show that phosphate-free P protein expressed in bacteria is transcriptionally inactive when reconstituted with L protein and viral N-RNA template free of cellular protein kinase. Phosphorylation of P protein by a cellular kinase(s) was essential for transcription as well as for further phosphorylation by an L-associated kinase, the two kinases acting in a sequential (cascade) manner. Phosphate groups introduced by cell kinase were stable, whereas those due to L kinase underwent a turnover which was coupled to ongoing transcription. We present a model for the phosphorylation pathway of P protein and propose that continued phosphorylation and dephosphorylation of P protein may represent a transcriptional regulatory (on-off) switch of nonsegmented negative-strand RNA viruses.

MeSH Terms
Animals Cell Line Cloning, Molecular Escherichia coli/genetics Kinetics Models, Biological Phosphoproteins/genetics,isolation & purification,metabolism Phosphorylation Protein Kinases/metabolism RNA-Dependent RNA Polymerase/genetics,isolation & purification,metabolism Recombinant Proteins/isolation & purification,metabolism Transcription, Genetic Transcriptional Activation Vesicular stomatitis Indiana virus/enzymology,genetics,metabolism
Chemicals
Phosphoproteins Recombinant Proteins Protein Kinases RNA-Dependent RNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Barik S
Department of Molecular Biology, Research Institute, Cleveland Clinic Foundation, Ohio 44195.
Banerjee A K
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.2.1109-1118.1992
Published
1992-02-00
Pages
1109-18
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC240815
Subset
IM
Grants
NIAID NIH HHS · AI 26585 · United States
External Links
PubMed source DOI: 10.1128/JVI.66.2.1109-1118.1992 PMC Full Text View journal details
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