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PMID: 12967982 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Brain-derived neurotrophic factor stimulates energy metabolism in developing cortical neurons.

The Journal of neuroscience : the official journal of the Society for Neuroscience ·Vol. 23 ·No. 23 ·2003-09-10 ·Pages 8212-20

Burkhalter J, Fiumelli H, Allaman I, Chatton JY, Martin JL

Abstract

Brain-derived neurotrophic factor (BDNF) promotes the biochemical and morphological differentiation of selective populations of neurons during development. In this study we examined the energy requirements associated with the effects of BDNF on neuronal differentiation. Because glucose is the preferred energy substrate in the brain, the effect of BDNF on glucose utilization was investigated in developing cortical neurons via biochemical and imaging studies. Results revealed that BDNF increases glucose utilization and the expression of the neuronal glucose transporter GLUT3. Stimulation of glucose utilization by BDNF was shown to result from the activation of Na+/K+-ATPase via an increase in Na+ influx that is mediated, at least in part, by the stimulation of Na+-dependent amino acid transport. The increased Na+-dependent amino acid uptake by BDNF is followed by an enhancement of overall protein synthesis associated with the differentiation of cortical neurons. Together, these data demonstrate the ability of BDNF to stimulate glucose utilization in response to an enhanced energy demand resulting from increases in amino acid uptake and protein synthesis associated with the promotion of neuronal differentiation by BDNF.

MeSH Terms
Amino Acids/metabolism,pharmacokinetics Animals Biological Transport/drug effects Brain-Derived Neurotrophic Factor/pharmacology Cells, Cultured Cerebral Cortex/cytology,embryology,metabolism Deoxyglucose/pharmacokinetics Energy Metabolism/drug effects Glucose/metabolism,pharmacokinetics Glucose Transporter Type 3 Kinetics Mice Monosaccharide Transport Proteins/genetics,metabolism Nerve Tissue Proteins Neurons/cytology,drug effects,metabolism Neuropeptide Y/biosynthesis Protein Biosynthesis RNA, Messenger/metabolism Sodium/metabolism Sodium-Potassium-Exchanging ATPase/metabolism
Chemicals
Amino Acids Brain-Derived Neurotrophic Factor Glucose Transporter Type 3 Monosaccharide Transport Proteins Nerve Tissue Proteins Neuropeptide Y RNA, Messenger Slc2a3 protein, mouse Deoxyglucose Sodium Sodium-Potassium-Exchanging ATPase Glucose
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Burkhalter Julia
Institute of Physiology, Faculty of Medicine, University of Lausanne, CH-1005 Lausanne, Switzerland.
Fiumelli Hubert
Allaman Igor
Chatton Jean-Yves
Martin Jean-Luc
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Article Info
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
Abbr.
J Neurosci
ISSN
1529-2401
Published
2003-09-10
Pages
8212-20
Language
English
Region
United States
NLM ID
8102140
PMCID
PMC6740684
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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