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PMID: 12953118 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A grape ASR protein involved in sugar and abscisic acid signaling.

The Plant cell ·Vol. 15 ·No. 9 ·2003-09-00 ·Pages 2165-80

Cakir B, Agasse A, Gaillard C, Saumonneau A, Delrot S, Atanassova R

Abstract

The function of ASR (ABA [abscisic acid]-, stress-, and ripening-induced) proteins remains unknown. A grape ASR, VvMSA, was isolated by means of a yeast one-hybrid approach using as a target the proximal promoter of a grape putative monosaccharide transporter (VvHT1). This promoter contains two sugar boxes, and its activity is induced by sucrose and glucose. VvMSA and VvHT1 share similar patterns of expression during the ripening of grape. Both genes are inducible by sucrose in grape berry cell culture, and sugar induction of VvMSA is enhanced strongly by ABA. These data suggest that VvMSA is involved in a common transduction pathway of sugar and ABA signaling. Gel-shift assays demonstrate a specific binding of VvMSA to the 160-bp fragment of the VvHT1 promoter and more precisely to two sugar-responsive elements present in this target. The positive regulation of VvHT1 promoter activity by VvMSA also is shown in planta by coexpression experiments. The nuclear localization of the yellow fluorescent protein-VvMSA fusion protein and the functionality of the VvMSA nuclear localization signal are demonstrated. Thus, a biological function is ascribed to an ASR protein. VvMSA acts as part of a transcription-regulating complex involved in sugar and ABA signaling.

MeSH Terms
Abscisic Acid/pharmacology Amino Acid Sequence Base Sequence Carbohydrates/pharmacology Cells, Cultured Cloning, Molecular Consensus Sequence/genetics DNA, Complementary/chemistry,genetics Gene Expression Regulation, Developmental/drug effects Gene Expression Regulation, Plant/drug effects Genome, Plant Molecular Sequence Data Nuclear Localization Signals/genetics Plant Proteins/genetics,metabolism Protein Interaction Mapping Sequence Analysis, DNA Sequence Homology, Amino Acid Signal Transduction Two-Hybrid System Techniques Vitis/drug effects,genetics,growth & development
Chemicals
Carbohydrates DNA, Complementary Nuclear Localization Signals Plant Proteins Abscisic Acid
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cakir Birsen
Unité Mixte de Recherche Centre National de la Recherche Scientifique 6161, Transport des Assimilats, Laboratoire de Physiologie, Biochimie et Biologie Moléculaire Végétales, Unité de Formation et de Recherches Sciences, 86022 Poitiers Cédex, France.
Agasse Alice
Gaillard Cécile
Saumonneau Amélie
Delrot Serge
Atanassova Rossitza
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.013854
Published
2003-09-00
Pages
2165-80
Language
English
Region
England
NLM ID
9208688
PMCID
PMC181338
Subset
IM
Databases
GENBANK
AF281656
External Links
PubMed source DOI: 10.1105/tpc.013854 PMC Full Text View journal details
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