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PMID: 12949119 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Irp9, encoded by the high-pathogenicity island of Yersinia enterocolitica, is able to convert chorismate into salicylate, the precursor of the siderophore yersiniabactin.

Journal of bacteriology ·Vol. 185 ·No. 18 ·2003-09-00 ·Pages 5648-53

Pelludat C, Brem D, Heesemann J

Abstract

The Irp9 protein of Yersinia enterocolitica participates in the synthesis of salicylate, the precursor of the siderophore yersiniabactin. In Pseudomonas species, salicylate synthesis is mediated by two enzymes: isochorismate synthase and isochorismate pyruvate-lyase. Both enzymes are required for complementation of a Yersinia irp9 mutant. However, irp9 is not able to complement Escherichia coli entC for the production of enterobactin, which requires isochorismate as a precursor. These results suggest that Irp9 directly converts chorismate into salicylate.

MeSH Terms
Bacterial Proteins/genetics,metabolism Chorismic Acid/metabolism Cyclohexenes Escherichia coli/genetics,metabolism Genetic Complementation Test Hydroxybenzoates/pharmacology Intramolecular Transferases/genetics,metabolism Lyases/genetics,metabolism Multigene Family Mutation Oxo-Acid-Lyases/genetics,metabolism Phenols Protein Precursors/metabolism Pseudomonas fluorescens/genetics Salicylates/metabolism Siderophores/metabolism Thiazoles Virulence/genetics Yersinia enterocolitica/drug effects,metabolism,pathogenicity
Chemicals
Bacterial Proteins Cyclohexenes Hydroxybenzoates Phenols Protein Precursors Salicylates Siderophores Thiazoles yersiniabactin chrome azurol S isochorismic acid Lyases salicylate synthetase Oxo-Acid-Lyases chorismate pyruvate lyase Intramolecular Transferases isochorismate synthase Chorismic Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pelludat Cosima
Institut für Mikrobiologie, D-BIOL, ETHZ, 8092 Zürich, Switzerland.
Brem Daniela
Heesemann Jürgen
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.185.18.5648-5653.2003
Published
2003-09-00
Pages
5648-53
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC193746
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.185.18.5648-5653.2003 PMC Full Text View journal details
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