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PMID: 12949112 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Complete genome sequence of the oral pathogenic Bacterium porphyromonas gingivalis strain W83.

Journal of bacteriology ·Vol. 185 ·No. 18 ·2003-09-00 ·Pages 5591-601

Nelson KE, Fleischmann RD, DeBoy RT, Paulsen IT, Fouts DE, Eisen JA, Daugherty SC, Dodson RJ, Durkin AS, Gwinn M, Haft DH, Kolonay JF, Nelson WC, Mason T, Tallon L, Gray J, Granger D, Tettelin H, Dong H, Galvin JL, Duncan MJ, Dewhirst FE, Fraser CM

Abstract

The complete 2,343,479-bp genome sequence of the gram-negative, pathogenic oral bacterium Porphyromonas gingivalis strain W83, a major contributor to periodontal disease, was determined. Whole-genome comparative analysis with other available complete genome sequences confirms the close relationship between the Cytophaga-Flavobacteria-Bacteroides (CFB) phylum and the green-sulfur bacteria. Within the CFB phyla, the genomes most similar to that of P. gingivalis are those of Bacteroides thetaiotaomicron and B. fragilis. Outside of the CFB phyla the most similar genome to P. gingivalis is that of Chlorobium tepidum, supporting the previous phylogenetic studies that indicated that the Chlorobia and CFB phyla are related, albeit distantly. Genome analysis of strain W83 reveals a range of pathways and virulence determinants that relate to the novel biology of this oral pathogen. Among these determinants are at least six putative hemagglutinin-like genes and 36 previously unidentified peptidases. Genome analysis also reveals that P. gingivalis can metabolize a range of amino acids and generate a number of metabolic end products that are toxic to the human host or human gingival tissue and contribute to the development of periodontal disease.

MeSH Terms
Bacterial Proteins/genetics,metabolism Biological Transport/genetics Genome, Bacterial Hemagglutinins/genetics Humans Molecular Sequence Data Mouth/microbiology Periodontal Diseases/microbiology Phylogeny Porphyromonas gingivalis/genetics,metabolism,pathogenicity Virulence/genetics
Chemicals
Bacterial Proteins Hemagglutinins
Authors & Affiliations
23 authors, click to expand affiliations / ORCID
Nelson Karen E
The Institute for Genomic Research, Rockville, Maryland 20850, USA. kenelson@tigr.org
Fleischmann Robert D
DeBoy Robert T
Paulsen Ian T
Fouts Derrick E
Eisen Jonathan A
Daugherty Sean C
Dodson Robert J
Durkin A Scott
Gwinn Michelle
Haft Daniel H
Kolonay James F
Nelson William C
Mason Tanya
Tallon Luke
Gray Jessica
Granger David
Tettelin Hervé
Dong Hong
Galvin Jamie L
Duncan Margaret J
Dewhirst Floyd E
Fraser Claire M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.185.18.5591-5601.2003
Email
kenelson@tigr.org
Published
2003-09-00
Pages
5591-601
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC193775
Subset
IM
Grants
NIDCR NIH HHS · R01DE-13914 · United States
Databases
GENBANK
AE015924
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1128/JB.185.18.5591-5601.2003 PMC Full Text View journal details
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