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PMID: 12945696 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Synthesis of fluorophore and quencher monomers for use in scorpion primers and nucleic acid structural probes.

Organic & biomolecular chemistry ·Vol. 1 ·No. 13 ·2003-07-07 ·Pages 2267-75

McKeen CM, Brown LJ, Nicol JT, Mellor JM, Brown T

Abstract

We report the syntheses of monomers to incorporate fluorescein and methyl red within the sequence and at the termini of modified oligonucleotides. These monomers have been used in the solid-phase synthesis of fluorogenic oligonucleotides for genetic analysis and the study of multi-stranded nucleic acid structures.

MeSH Terms

Azo Compounds/chemical synthesis,chemistry DNA Primers/chemistry,genetics Fluorescein/chemical synthesis,chemistry Fluorescent Dyes/chemical synthesis,chemistry Models, Biological Models, Chemical Nucleic Acid Probes/chemistry Oligonucleotides/chemical synthesis,chemistry Polymerase Chain Reaction

Chemicals

Azo Compounds DNA Primers Fluorescent Dyes Nucleic Acid Probes Oligonucleotides methyl red Fluorescein

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

McKeen Catherine M

Department of Chemistry, University of Southampton, Highfield, Southampton, UK S017 1BJ.

Brown Lynda J

Nicol Jamie T G

Mellor John M

Brown Tom

Article Info

Journal

Organic & biomolecular chemistry

Abbr.

Org Biomol Chem

ISSN

1477-0520

DOI

10.1039/b301859h

Published

2003-07-07

Pages

2267-75

Language

English

Region

England

NLM ID

101154995

Subset

External Links

PubMed source DOI: 10.1039/b301859h View journal details

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Synthesis of fluorophore and quencher monomers for use in scorpion primers and nucleic acid structural probes.

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