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PMID: 12941923 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Analysis of early human immunodeficiency virus type 1 DNA synthesis by use of a new sensitive assay for quantifying integrated provirus.

Journal of virology ·Vol. 77 ·No. 18 ·2003-09-00 ·Pages 10119-24

Brussel A, Sonigo P

Abstract

A novel Alu-long terminal repeat (LTR)-based real-time nested-PCR assay was developed to quantify integrated human immunodeficiency virus type 1 (HIV-1) DNA in infected cells with both accuracy and high sensitivity (six proviruses within 50,000 cell equivalents). Parallel assays for total HIV-1 DNA and two-LTR HIV-1 DNA circles allowed the synthesis and fate of the different HIV-1 DNA species to be monitored upon a single round of viral replication.

MeSH Terms
DNA, Viral/biosynthesis HIV Long Terminal Repeat HIV-1/genetics Humans Polymerase Chain Reaction Sensitivity and Specificity Virus Integration
Chemicals
DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brussel Audrey
Département des Maladies Infectieuses, Institut Cochin, INSERM U567, CNRS UMR 8104, Université René Descartes, 22 rue Méchain, 75014 Paris, France.
Sonigo Pierre
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.77.18.10119-10124.2003
Published
2003-09-00
Pages
10119-24
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC224570
Subset
IM
External Links
PubMed source DOI: 10.1128/jvi.77.18.10119-10124.2003 PMC Full Text View journal details
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