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PMID: 12923103 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Efflux of cytoplasmically acting antibiotics from gram-negative bacteria: periplasmic substrate capture by multicomponent efflux pumps inferred from their cooperative action with single-component transporters.

Journal of bacteriology ·Vol. 185 ·No. 17 ·2003-09-00 ·Pages 5287-9

Palmer M

Abstract

In gram-negative bacteria, coexpression of single- and multicomponent efflux pumps may result in multiplicative enhancement of the level of resistance against cytoplasmically acting antibiotics. Here, a simple model is presented to show that this cooperative effect can be accounted for only if substrate capture by the multicomponent efflux transporter occurs in the periplasm but not the cytosol.

MeSH Terms

Anti-Bacterial Agents/metabolism,pharmacology Bacterial Proteins/metabolism Cell Membrane/metabolism Cytoplasm/metabolism Cytosol/metabolism Drug Resistance, Bacterial Gram-Negative Bacteria/drug effects Membrane Transport Proteins/metabolism Models, Biological Periplasm/metabolism

Chemicals

Anti-Bacterial Agents Bacterial Proteins Membrane Transport Proteins

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Palmer Michael

Department of Chemistry, University of Waterloo, Waterloo, Ontario N2L 3G1, Canada. mpalmer@uwaterloo.ca

References (14)

14 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/JB.185.17.5287-5289.2003

mpalmer@uwaterloo.ca

Published

2003-09-00

Pages

5287-9

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC181015

Subset

External Links

PubMed source DOI: 10.1128/JB.185.17.5287-5289.2003 PMC Full Text View journal details

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Efflux of cytoplasmically acting antibiotics from gram-negative bacteria: periplasmic substrate capture by multicomponent efflux pumps inferred from their cooperative action with single-component transporters.

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