Abstract
Peptide mass-signature genotyping (PMSG) is a scanning genotyping method that identifies mutations and polymorphisms by translating the sequence of interest in more than one reading frame and measuring the masses of the resulting peptides by mass spectrometry. PMSG was applied to the RDS/peripherin gene of 16 individuals from a family exhibiting autosomal dominant macular degeneration. The method revealed an A-->T transversion in the 5' splice site of intron 2 that is the likely cause of the disease. It also revealed four different minihaplotypes in exon 3 that represent particular combinations of SNPs at four different locations. This study demonstrates the utility of PMSG for identifying and characterizing point mutations and local minihaplotypes that are not readily analyzed by other approaches.
MeSH Terms
DNA Mutational Analysis/methods
Eye Proteins/chemistry,genetics
Female
Genotype
Haplotypes
Humans
Intermediate Filament Proteins/chemistry,genetics
Male
Membrane Glycoproteins/chemistry,genetics
Mutation
Nerve Tissue Proteins/chemistry,genetics
Pedigree
Peptides/chemistry,genetics
Peripherins
Retinal Degeneration/genetics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
Chemicals
Eye Proteins
Intermediate Filament Proteins
Membrane Glycoproteins
Nerve Tissue Proteins
PRPH protein, human
PRPH2 protein, human
Peptides
Peripherins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Telmer Cheryl A
SpectraGenetics LLC, Pittsburgh, Pennsylvania 15213, USA. cheryl.telmer@spectragenetics.com
Retchless Adam C
Kinsey Ashley D
Conley Yvette
Rigatti Brian
Gorin Michael B
Jarvik Jonathan W
Retchless Adam R
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