Home LiteratureArticle Details
PMID: 12883906 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Dissociation of mammalian Polycomb-group proteins, Ring1B and Rae28/Ph1, from the chromatin correlates with configuration changes of the chromatin in mitotic and meiotic prophase.

Histochemistry and cell biology ·Vol. 120 ·No. 2 ·2003-08-00 ·Pages 111-9

Miyagishima H, Isono K, Fujimura Y, Iyo M, Takihara Y, Masumoto H, Vidal M, Koseki H

Abstract

The Polycomb group (PcG) gene products form complexes that regulate chromatin configuration to mediate cellular memory to postmitotic somatic cells and postmeiotic oocytes in Drosophila melanogaster. Structural and functional similarities of PcG proteins between invertebrates and vertebrates suggest mammalian PcG proteins may be involved to imprint transcriptional status at various loci into postmitotic and postmeiotic daughter cells. To address molecular mechanisms underlying PcG-mediated cellular memory, it might be a prerequisite to understand subcellular localization of PcG proteins during mitosis and meiosis. In this study, we analyzed subcellular localization of Rae28/Ph1 and Ring1B by using newly generated monoclonal antibodies in mitotic somatic cells and meiotic mouse oocytes. Results suggest that Rae28/Ph1 and Ring1B dissociate from the chromatin upon its condensation in mitotic prophase in the U2-OS human osteosarcoma cell line. During maturation of oocytes, significant alterations of Rae28/Ph1 and Ring1B localization are concordant with configuration changes of the chromatin at the germinal vesicle stage of meiotic prophase. Importantly, dissociation of Rae28/Ph1 and Ring1B from the chromatin temporally correlates with transcriptional arrest both in mitosis and meiosis. Present and previous observations suggest molecular mechanisms required for mitotic regulation of RNA polymerase II could be involved in dissociation of PcG proteins.

MeSH Terms
Animals Antibodies, Monoclonal/metabolism Blotting, Western Carrier Proteins Cell Cycle Proteins/analysis,metabolism Cell Line, Tumor Chromatin/chemistry,metabolism,ultrastructure Drosophila Proteins/analysis,metabolism Homeodomain Proteins/analysis,genetics,metabolism Humans Meiosis Mice Mitosis Oocytes/chemistry Polycomb Repressive Complex 1 Polycomb-Group Proteins Prophase Protein Binding Repressor Proteins/analysis,classification,metabolism
Chemicals
Antibodies, Monoclonal Carrier Proteins Cell Cycle Proteins Chromatin Drosophila Proteins Homeodomain Proteins PHC1 protein, human Pc protein, Drosophila Phc1 protein, mouse Polycomb-Group Proteins Repressor Proteins Polycomb Repressive Complex 1
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Miyagishima Hiro
Department of Molecular Embryology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuoku, 260-8670 Chiba, Japan.
Isono Kyoichi
Fujimura Yuichi
Iyo Masaomi
Takihara Yoshihiro
Masumoto Hiroshi
Vidal Miguel
Koseki Haruhiko
References (45)
45 references, click to expand
  1. Phosphorylation of the carboxy-terminal repeat domain in RNA polymerase II by cyclin-dependent kinases is sufficient to inhibit transcription.
    J Cell Biochem. 1997 Mar 1;64(3):390-402 PMID: 9057097
  2. Homeotic transformations of the axial skeleton that accompany a targeted deletion of E2f6.
    EMBO Rep. 2002 Jul;3(7):695-700 PMID: 12101104
  3. Ring1A is a transcriptional repressor that interacts with the Polycomb-M33 protein and is expressed at rhombomere boundaries in the mouse hindbrain.
    EMBO J. 1997 Oct 1;16(19):5930-42 PMID: 9312051
  4. Genes affecting the segmental subdivision of the Drosophila embryo.
    Cold Spring Harb Symp Quant Biol. 1985;50:145-54 PMID: 3868475
  5. Isolation and characterization of retinoic acid-inducible cDNA clones in F9 cells: one of the early inducible clones encodes a novel protein sharing several highly homologous regions with a Drosophila polyhomeotic protein.
    Differentiation. 1994 Jun;57(1):39-50 PMID: 8070621
  6. A role for mel-18, a Polycomb group-related vertebrate gene, during theanteroposterior specification of the axial skeleton.
    Development. 1996 May;122(5):1513-22 PMID: 8625838
  7. The human polycomb group complex associates with pericentromeric heterochromatin to form a novel nuclear domain.
    J Cell Biol. 1998 Aug 24;142(4):887-98 PMID: 9722603
  8. Targeted disruption of the mouse homologue of the Drosophila polyhomeotic gene leads to altered anteroposterior patterning and neural crest defects.
    Development. 1997 Oct;124(19):3673-82 PMID: 9367423
  9. Loss- and gain-of-function mutations show a polycomb group function for Ring1A in mice.
    Development. 2000 Dec;127(23 ):5093-100 PMID: 11060235
  10. Control of the replicative life span of human fibroblasts by p16 and the polycomb protein Bmi-1.
    Mol Cell Biol. 2003 Jan;23 (1):389-401 PMID: 12482990
  11. Posterior transformation, neurological abnormalities, and severe hematopoietic defects in mice with a targeted deletion of the bmi-1 proto-oncogene.
    Genes Dev. 1994 Apr 1;8(7):757-69 PMID: 7926765
  12. Production of monoclonal antibodies against mammalian Ring1B proteins.
    Hybridoma. 2001 Feb;20(1):43-6 PMID: 11289226
  13. Native and recombinant polycomb group complexes establish a selective block to template accessibility to repress transcription in vitro.
    Mol Cell Biol. 2002 Nov;22(22):7919-28 PMID: 12391159
  14. Expression of novel DNA-binding protein with zinc finger structure in various tumor cells.
    J Biol Chem. 1990 Nov 15;265(32):20021-6 PMID: 2246278
  15. Interaction of mouse polycomb-group (Pc-G) proteins Enx1 and Enx2 with Eed: indication for separate Pc-G complexes.
    Mol Cell Biol. 1998 Jun;18(6):3572-9 PMID: 9584197
  16. The Bmi-1 oncoprotein interacts with dinG and MPh2: the role of RING finger domains.
    Oncogene. 1998 May 14;16(19):2541-7 PMID: 9627119
  17. An evaluation of the double thymidine block for synchronizing mammalian cells at the G1-S border.
    Exp Cell Res. 1971 Sep;68(1):163-8 PMID: 5165443
  18. Identification of cooperating oncogenes in E mu-myc transgenic mice by provirus tagging.
    Cell. 1991 May 31;65(5):737-52 PMID: 1904008
  19. Involvement of the Polycomb-group gene Ring1B in the specification of the anterior-posterior axis in mice.
    Development. 2002 Sep;129(18):4171-83 PMID: 12183370
  20. Altered cellular proliferation and mesoderm patterning in Polycomb-M33-deficient mice.
    Development. 1997 Feb;124(3):721-9 PMID: 9043087
  21. The mouse has a Polycomb-like chromobox gene.
    Development. 1992 Apr;114(4):921-9 PMID: 1352241
  22. Propagating memory of transcriptional states.
    Trends Genet. 1995 Aug;11(8):295-7 PMID: 8585123
  23. RING1 is associated with the polycomb group protein complex and acts as a transcriptional repressor.
    Mol Cell Biol. 1997 Jul;17 (7):4105-13 PMID: 9199346
  24. Interference with the expression of a novel human polycomb protein, hPc2, results in cellular transformation and apoptosis.
    Mol Cell Biol. 1997 Oct;17 (10 ):6076-86 PMID: 9315667
  25. Stabilization of chromatin structure by PRC1, a Polycomb complex.
    Cell. 1999 Jul 9;98 (1):37-46 PMID: 10412979
  26. Mammalian homologues of the Polycomb-group gene Enhancer of zeste mediate gene silencing in Drosophila heterochromatin and at S. cerevisiae telomeres.
    EMBO J. 1997 Jun 2;16(11):3219-32 PMID: 9214638
  27. Set domain-containing protein, G9a, is a novel lysine-preferring mammalian histone methyltransferase with hyperactivity and specific selectivity to lysines 9 and 27 of histone H3.
    J Biol Chem. 2001 Jul 6;276(27):25309-17 PMID: 11316813
  28. Chromatin-silencing mechanisms in Drosophila maintain patterns of gene expression.
    Trends Genet. 1997 Aug;13(8):314-8 PMID: 9260517
  29. Mitotic repression of RNA polymerase II transcription is accompanied by release of transcription elongation complexes.
    Mol Cell Biol. 1997 Oct;17 (10 ):5791-802 PMID: 9315637
  30. Identification and characterization of interactions between the vertebrate polycomb-group protein BMI1 and human homologs of polyhomeotic.
    Mol Cell Biol. 1997 Apr;17 (4):2326-35 PMID: 9121482
  31. The molecular mechanism of mitotic inhibition of TFIIH is mediated by phosphorylation of CDK7.
    Genes Dev. 1998 Nov 15;12(22):3541-50 PMID: 9832506
  32. Positional cloning of a global regulator of anterior-posterior patterning in mice.
    Nature. 1996 Sep 19;383(6597):250-3 PMID: 8805699
  33. A complex with chromatin modifiers that occupies E2F- and Myc-responsive genes in G0 cells.
    Science. 2002 May 10;296(5570):1132-6 PMID: 12004135
  34. MBLR, a new RING finger protein resembling mammalian Polycomb gene products, is regulated by cell cycle-dependent phosphorylation.
    Genes Cells. 2002 Aug;7(8):835-50 PMID: 12167161
  35. The E2F6 transcription factor is a component of the mammalian Bmi1-containing polycomb complex.
    Proc Natl Acad Sci U S A. 2001 Feb 13;98 (4):1519-24 PMID: 11171983
  36. Pc-G/trx-G and the SWI/SNF connection: developmental gene regulation through chromatin remodeling.
    Genesis. 2000 Mar;26(3):189-97 PMID: 10705379
  37. M33, a mammalian homologue of Drosophila Polycomb localises to euchromatin within interphase nuclei but is enriched within the centromeric heterochromatin of metaphase chromosomes.
    Cytogenet Cell Genet. 1997;78(1):50-5 PMID: 9345907
  38. Chromatin-association of the Polycomb group protein BMI1 is cell cycle-regulated and correlates with its phosphorylation status.
    J Cell Sci. 1999 Dec;112 ( Pt 24):4627-39 PMID: 10574711
  39. Repression of RNA polymerase II and III transcription during M phase of the cell cycle.
    Exp Cell Res. 1996 Dec 15;229(2):282-8 PMID: 8986611
  40. Identification and analysis of a third mouse Polycomb gene, MPc3.
    Gene. 2000 Jan 25;242(1-2):31-40 PMID: 10721694
  41. Polycomb and polyhomeotic are constituents of a multimeric protein complex in chromatin of Drosophila melanogaster.
    EMBO J. 1992 Aug;11(8):2941-50 PMID: 1353445
  42. pipsqueak encodes a factor essential for sequence-specific targeting of a polycomb group protein complex.
    Mol Cell Biol. 2002 Sep;22(17 ):6261-71 PMID: 12167718
  43. Differential transcriptional activity associated with chromatin configuration in fully grown mouse germinal vesicle oocytes.
    Biol Reprod. 1999 Mar;60(3):580-7 PMID: 10026102
  44. Tumor suppression at the mouse INK4a locus mediated by the alternative reading frame product p19ARF.
    Cell. 1997 Nov 28;91(5):649-59 PMID: 9393858
  45. Mitotic regulation of TFIID: inhibition of activator-dependent transcription and changes in subcellular localization.
    Genes Dev. 1996 Oct 1;10 (19):2389-400 PMID: 8843192
Article Info
Journal
Histochemistry and cell biology
Abbr.
Histochem Cell Biol
ISSN
0948-6143
DOI
10.1007/s00418-003-0551-2
Published
2003-08-00
Epub
2003-00-22
Pages
111-9
Language
English
Region
Germany
NLM ID
9506663
Subset
IM
External Links
PubMed source DOI: 10.1007/s00418-003-0551-2 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2