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PMID: 12867465 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Phase variation in the phage growth limitation system of Streptomyces coelicolor A3(2).

Journal of bacteriology ·Vol. 185 ·No. 15 ·2003-08-00 ·Pages 4558-63

Sumby P, Smith MC

Abstract

The phase-variable phage growth limitation (Pgl) system of Streptomyces coelicolor A3(2) is an unusual bacteriophage resistance mechanism that confers protection against the temperate phage phiC31 and homoimmune relatives. Pgl is subject to phase variation, and data presented here show that this is at least partially due to expansion and contraction of a polyguanine tract present within the putative adenine-specific DNA methyltransferase gene, pglX. Furthermore, the pglX paralogue SC6G9.02, here renamed pglS, was shown to be able to interfere with the Pgl phenotype, suggesting that PglS could provide an alternative activity to that conferred by PglX.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics,metabolism Bacteriophages/physiology Carrier Proteins/genetics,metabolism Intracellular Signaling Peptides and Proteins Molecular Sequence Data Mutation Phenotype Site-Specific DNA-Methyltransferase (Adenine-Specific) Streptomyces/genetics,growth & development,virology Viral Plaque Assay
Chemicals
Bacterial Proteins Carrier Proteins Intracellular Signaling Peptides and Proteins PglY protein, Streptomyces coelicolor PglZ protein, Streptomyces coelicolor Site-Specific DNA-Methyltransferase (Adenine-Specific)
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sumby Paul
Institute of Genetics, University of Nottingham, Queens Medical Centre, Nottingham, NG7 2UH, United Kingdom.
Smith Margaret C M
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.185.15.4558-4563.2003
Published
2003-08-00
Pages
4558-63
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC165769
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.185.15.4558-4563.2003 PMC Full Text View journal details
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