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PMID: 12867439 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

PlmA, a new member of the GntR family, has plasmid maintenance functions in Anabaena sp. strain PCC 7120.

Journal of bacteriology ·Vol. 185 ·No. 15 ·2003-08-00 ·Pages 4315-25

Lee MH, Scherer M, Rigali S, Golden JW

Abstract

The filamentous cyanobacterium Anabaena (Nostoc) sp. strain PCC 7120 maintains a genome that is divided into a 6.4-Mb chromosome, three large plasmids of more that 100 kb, two medium-sized plasmids of 55 and 40 kb, and a 5.5-kb plasmid. Plasmid copy number can be dynamic in some cyanobacterial species, and the genes that regulate this process have not been characterized. Here we show that mutations in an open reading frame, all1076, reduce the numbers of copies per chromosome of several plasmids. In a mutant strain, plasmids pCC7120delta and pCC7120zeta are both reduced to less than 50% of their wild-type levels. The exogenous pDU1-based plasmid pAM1691 is reduced to less than 25% of its wild-type level, and the plasmid is rapidly lost. The peptide encoded by all1076 shows similarity to members of the GntR family of transcriptional regulators. Phylogenetic analysis reveals a new domain topology within the GntR family. PlmA homologs, all coming from cyanobacterial species, form a new subfamily that is distinct from the previously identified subfamilies. The all1076 locus, named plmA, regulates plasmid maintenance functions in Anabaena sp. strain PCC 7120.

MeSH Terms
Anabaena/genetics,growth & development Bacterial Proteins/classification,genetics,metabolism Culture Media DNA-Binding Proteins/classification,genetics Escherichia coli Proteins/classification,genetics Gene Dosage Gene Expression Regulation, Bacterial Genes, Bacterial Glutamate-Ammonia Ligase/genetics,metabolism Mutation Phylogeny Plasmids/genetics Polymerase Chain Reaction Repressor Proteins/classification,genetics Transcription Factors/classification,genetics,metabolism
Chemicals
Bacterial Proteins Culture Media DNA-Binding Proteins Escherichia coli Proteins GntR protein, E coli PatS protein, Anabaena Repressor Proteins Transcription Factors glutamine synthetase I Glutamate-Ammonia Ligase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lee Martin H
Department of Biology, Texas A&M University, College Station, Texas 77843-3258, USA.
Scherer Michael
Rigali Sébastien
Golden James W
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.185.15.4315-4325.2003
Published
2003-08-00
Pages
4315-25
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC165748
Subset
IM
Grants
NINDS NIH HHS · P01 NS039546 · United States
NIGMS NIH HHS · R01 GM036890 · United States
NIGMS NIH HHS · GM36890 · United States
External Links
PubMed source DOI: 10.1128/JB.185.15.4315-4325.2003 PMC Full Text View journal details
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