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PMID: 1279521 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Temperature-dependent template switching during in vitro cDNA synthesis by the AMV-reverse transcriptase.

Nucleic acids research ·Vol. 20 ·No. 20 ·1992-10-25 ·Pages 5443-50

Ouhammouch M, Brody EN

Abstract

Reverse transcriptase template switching has been invoked to explain several aspects of retroviral replication and recombination, and has been reported in vitro for the Moloney murine leukemia virus (M-MuLV) reverse transcriptase. During in vitro cDNA synthesis, the avian myeloblastosis virus (AMV) reverse transcriptase can switch from one template to another in a homology-dependent and temperature-dependent manner. Chimeric cDNA molecules are generated within 30 min at high incubation temperatures, with an increasing efficiency from 42 degrees C to 50 degrees C. Such products are detectable only after much longer incubation times when primer extension reactions are carried out at lower temperatures (90 min at 37 degrees C).

MeSH Terms

Avian Myeloblastosis Virus/enzymology Base Sequence DNA/biosynthesis DNA-Binding Proteins Escherichia coli/genetics Molecular Sequence Data Oligodeoxyribonucleotides/genetics Plasmids/genetics RNA, Messenger/metabolism RNA-Directed DNA Polymerase/metabolism Sequence Homology, Nucleic Acid Temperature Templates, Genetic Transcription Factors/genetics Viral Proteins/genetics

Chemicals

DNA-Binding Proteins MotA protein, Enterobacteria phage T4 Oligodeoxyribonucleotides RNA, Messenger Transcription Factors Viral Proteins DNA RNA-Directed DNA Polymerase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ouhammouch M

CNRS Centre de Génétique Moléculaire, Laboratoire Propre Associé à l'Université Pierre et Marie Curie, Gif-sur-Yvette, France.

Brody E N

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14 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/20.20.5443

Published

1992-10-25

Pages

5443-50

Language

English

Region

England

NLM ID

0411011

PMCID

PMC334354

Subset

External Links

PubMed source DOI: 10.1093/nar/20.20.5443 PMC Full Text View journal details

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Temperature-dependent template switching during in vitro cDNA synthesis by the AMV-reverse transcriptase.

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