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PMID: 12782738 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

HCF152, an Arabidopsis RNA binding pentatricopeptide repeat protein involved in the processing of chloroplast psbB-psbT-psbH-petB-petD RNAs.

The Plant cell ·Vol. 15 ·No. 6 ·2003-06-00 ·Pages 1480-95

Meierhoff K, Felder S, Nakamura T, Bechtold N, Schuster G

Abstract

The psbB-psbT-psbH-petB-petD operon of higher plant chloroplasts is a heterogeneously composed transcriptional unit that undergoes complex RNA processing events until the mature oligocistronic RNAs are formed. To identify the nucleus-encoded factors required for the processing and expression of psbB-psbT-psbH-petB-petD transcripts, we performed mutational analysis using Arabidopsis. The allelic nuclear mutants hcf152-1 and hcf152-2 were identified that are affected specifically in the accumulation of the plastidial cytochrome b(6)f complex. In both mutants, reduced amounts of spliced petB RNAs (encoding the cytochrome b(6) subunit) were detected, thus explaining the observed protein deficiencies. Additionally, mutant hcf152-1 is affected in the accumulation of transcripts cleaved between the genes psbH and petB. As a result of a close T-DNA insertion, the HCF152 gene was cloned and its identity confirmed by complementation of homozygous mutant plants. HCF152 encodes a pentatricopeptide repeat (PPR) protein with 12 putative PPR motifs that is located inside the chloroplast. The protein shows a significant structural, but not primary, sequence similarity to the maize protein CRP1, which is involved in the processing and translation of the chloroplast petD and petA RNAs. In addition, we found that HCF152 is an RNA binding protein that binds certain areas of the petB transcript. The protein possibly exists in the chloroplast as a homodimer and is not associated with other proteins to form a high molecular mass complex.

MeSH Terms
Alleles Amino Acid Sequence Arabidopsis/genetics,metabolism Arabidopsis Proteins/genetics,metabolism Chlorophyll/metabolism Cytochrome b Group/genetics Cytochrome b6f Complex Electron Transport/genetics Gene Expression Regulation, Plant/radiation effects Genes, Plant/genetics Light Light-Harvesting Protein Complexes Molecular Sequence Data Mutation Operon Phosphoproteins/genetics Photosynthetic Reaction Center Complex Proteins/genetics,metabolism Photosystem II Protein Complex Plant Proteins/genetics Protein Binding RNA Processing, Post-Transcriptional RNA, Chloroplast/genetics,metabolism RNA-Binding Proteins/genetics,metabolism Repetitive Sequences, Amino Acid/genetics Sequence Homology, Amino Acid
Chemicals
Arabidopsis Proteins Cytochrome b Group HCF152 protein, Arabidopsis Light-Harvesting Protein Complexes PetB protein, plant Phosphoproteins Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Proteins RNA, Chloroplast RNA-Binding Proteins petD protein, cytochrome b(6)f complex photosystem II, psbH subunit Chlorophyll Cytochrome b6f Complex
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Meierhoff Karin
Heinrich-Heine-Universität, Institut für Entwicklungs und Molekularbiologie der Pflanzen, 40225 Düsseldorf, Germany. karin.meierhoff@uni-duesseldorf.de
Felder Susanne
Nakamura Takahiro
Bechtold Nicole
Schuster Gadi
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.010397
Email
karin.meierhoff@uni-duesseldorf.de
Published
2003-06-00
Pages
1480-95
Language
English
Region
England
NLM ID
9208688
PMCID
PMC156381
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.010397 PMC Full Text View journal details
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