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PMID: 12767999 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Tomato yellow leaf curl Sardinia virus rep-derived resistance to homologous and heterologous geminiviruses occurs by different mechanisms and is overcome if virus-mediated transgene silencing is activated.

Journal of virology ·Vol. 77 ·No. 12 ·2003-06-00 ·Pages 6785-98

Lucioli A, Noris E, Brunetti A, Tavazza R, Ruzza V, Castillo AG, Bejarano ER, Accotto GP, Tavazza M

Abstract

The replication-associated protein (Rep) of geminiviruses is involved in several biological processes brought about by the presence of distinct functional domains. Recently, we have exploited the multifunctional character of the Tomato yellow leaf curl Sardinia virus (TYLCSV) Rep to develop a molecular interference strategy to impair TYLCSV infection. We showed that transgenic expression of its N-terminal 210 amino acids (Rep-210) confers resistance to the homologous virus by inhibiting viral transcription and replication. We have now used biochemical and transgenic approaches to carry out a fuller investigation of the molecular resistance mechanisms in transgenic plants expressing Rep-210. We show that Rep-210 confers resistance through two distinct molecular mechanisms, depending on the challenging virus. Resistance to the homologous virus is achieved by the ability of Rep-210 to tightly inhibit C1 gene transcription, while that to heterologous virus is due to the interacting property of the Rep-210 oligomerization domain. Furthermore, we present evidence that in Rep-210-expressing plants, the duration of resistance is related to the ability of the challenging virus to shut off transgene expression by a posttranscriptional homology-dependent gene silencing mechanism. A model of Rep-210-mediated geminivirus resistance that takes transgene- and virus-mediated mechanisms into account is proposed.

MeSH Terms
Base Sequence DNA Helicases/chemistry,genetics,metabolism DNA-Binding Proteins Down-Regulation Geminiviridae/genetics,pathogenicity Lycopersicon esculentum/microbiology Molecular Sequence Data Mutation Plant Diseases/microbiology Plants, Genetically Modified RNA Interference Tobacco/virology Trans-Activators/chemistry,genetics,metabolism Transcription, Genetic Transgenes Viral Proteins/chemistry,genetics,metabolism Virus Replication
Chemicals
DNA-Binding Proteins Trans-Activators Viral Proteins replication initiator protein DNA Helicases
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Lucioli Alessandra
ENEA C.R. Casaccia, Settore Biotec, 00060 Rome, Italy.
Noris Emanuela
Brunetti Angela
Tavazza Raffaela
Ruzza Valentino
Castillo Araceli G
Bejarano Eduardo R
Accotto Gian Paolo
Tavazza Mario
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/jvi.77.12.6785-6798.2003
Published
2003-06-00
Pages
6785-98
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC156158
Subset
IM
External Links
PubMed source DOI: 10.1128/jvi.77.12.6785-6798.2003 PMC Full Text View journal details
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