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PMID: 1272801 Published · ppublish English Journal Article

The binding of poly (rA) and poly (rU) to denatured DNA. II. Studies with natural DNAs.

Nucleic acids research ·Vol. 3 ·No. 4 ·1976-04-00 ·Pages 1029-51

Mol JN, Borst P

Abstract

We have studied the interaction of poly(rA) and poly(rU) with natural DNAs containing (dA.dT)n sequences. The results indicate that hybridization of poly(rA) to denatured DNA can be used to estimate the size and frequency of large (dA.dT)n tracts, whereas hybridization with poly(rU) does not give reliable information on these points. In 6.6 M CsCl, poly(rU) can form stable complexes with denatured DNA containing short (dA)n tracts (n less than or equal to 6), whereas binding of poly(rA) to denatured DNA under these conditions requires much larger (dT)n tracts (estimated n greater than 13). Moreover, binding of poly(rA) requires pre-hybridization in low salt, because free poly(rA) precipitates in 6.6 M CsCl.

MeSH Terms
Base Sequence Binding Sites Cell Nucleus/analysis Centrifugation, Density Gradient DNA DNA, Bacterial DNA, Mitochondrial Dictyostelium Kinetics Molecular Weight Nucleic Acid Conformation Nucleic Acid Denaturation Nucleic Acid Hybridization Poly A Poly U Ribonucleases Structure-Activity Relationship Temperature
Chemicals
DNA, Bacterial DNA, Mitochondrial Poly A Poly U DNA Ribonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Mol J N
Borst P
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30 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/3.4.1029
Published
1976-04-00
Pages
1029-51
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342963
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/3.4.1029 PMC Full Text View journal details
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