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PMID: 12724408 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Two ubiquitin-conjugating enzymes, UbcP1/Ubc4 and UbcP4/Ubc11, have distinct functions for ubiquitination of mitotic cyclin.

Molecular and cellular biology ·Vol. 23 ·No. 10 ·2003-05-00 ·Pages 3497-505

Seino H, Kishi T, Nishitani H, Yamao F

Abstract

Cell cycle events are regulated by sequential activation and inactivation of Cdk kinases. Mitotic exit is accomplished by the inactivation of mitotic Cdk kinase, which is mainly achieved by degradation of cyclins. The ubiquitin-proteasome system is involved in this process, requiring APC/C (anaphase-promoting complex/cyclosome) as a ubiquitin ligase. In Xenopus and clam oocytes, the ubiquitin-conjugating enzymes that function with APC/C have been identified as two proteins, UBC4 and UBCx/E2-C. Previously we reported that the fission yeast ubiquitin-conjugating enzyme UbcP4/Ubc11, a homologue of UBCx/E2-C, is required for mitotic transition. Here we show that the other fission yeast ubiquitin-conjugating enzyme, UbcP1/Ubc4, which is homologous to UBC4, is also required for mitotic transition in the same manner as UbcP4/Ubc11. Both ubiquitin-conjugating enzymes are essential for cell division and directly required for the degradation of mitotic cyclin Cdc13. They function nonredundantly in the ubiquitination of CDC13 because a defect in ubcP1/ubc4+ cannot be suppressed by high expression of UbcP4/Ubc11 and a defect in ubcP4/ubc11+ cannot be suppressed by high expression of UbcP1/Ubc4. In vivo analysis of the ubiquitinated state of Cdc13 shows that the ubiquitin chains on Cdc13 were short in ubcP1/ubc4 mutant cells while ubiquitinated Cdc13 was totally reduced in ubcP4/ubc11 mutant cells. Taken together, these results indicate that the two ubiquitin-conjugating enzymes play distinct and essential roles in the degradation of mitotic cyclin Cdc13, with the UbcP4/Ubc11-pathway initiating ubiquitination of Cdc13 and the UbcP1/Ubc4-pathway elongating the short ubiquitin chains on Cdc13.

MeSH Terms
Amino Acid Sequence Animals Blotting, Western Carrier Proteins/physiology Cyclin B/metabolism Cyclins/metabolism Ligases/physiology Mitosis Molecular Sequence Data Mutation Oocytes/metabolism Plasmids/metabolism Protein Binding Saccharomyces cerevisiae Proteins/physiology Sequence Homology, Amino Acid Temperature Time Factors Ubiquitin/metabolism Ubiquitin-Conjugating Enzymes Xenopus
Chemicals
Carrier Proteins Cyclin B Cyclins Saccharomyces cerevisiae Proteins Ubiquitin UBC11 protein, S cerevisiae Ubiquitin-Conjugating Enzymes ubiquitin-conjugating enzyme UBC4 Ligases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Seino Hiroaki
Division of Mutagenesis, National Institute of Genetics, Mishima, Shizuoka 411-8540, Japan. hseino@lab.nig.ac.jp
Kishi Tsutomu
Nishitani Hideo
Yamao Fumiaki
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.23.10.3497-3505.2003
Email
hseino@lab.nig.ac.jp
Published
2003-05-00
Pages
3497-505
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC164763
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.23.10.3497-3505.2003 PMC Full Text View journal details
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