Abstract
The organic cation transporter, OCT1, is a major hepatic transporter that mediates the uptake of many organic cations from the blood into the liver where the compounds may be metabolized or secreted into the bile. Because OCT1 interacts with a variety of structurally diverse organic cations, including clinically used drugs as well as toxic substances (e.g., N-methylpyridinium, MPP(+)), it is an important determinant of systemic exposure to many xenobiotics. To understand the genetic basis of extensive interindividual differences in xenobiotic disposition, we functionally characterized 15 protein-altering variants of the human liver organic cation transporter, OCT1, in Xenopus oocytes. All variants that reduced or eliminated function (OCT1-R61C, OCT1-P341L, OCT1-G220V, OCT1-G401S, and OCT1-G465R) altered evolutionarily conserved amino acid residues. In general, variants with decreased function had amino acid substitutions that resulted in more radical chemical changes (higher Grantham values) and were less evolutionarily favorable (lower blosum62 values) than variants that maintained function. A variant with increased function (OCT1-S14F) changed an amino acid residue such that the human protein matched the consensus of the OCT1 mammalian orthologs. Our results indicate that changes at evolutionarily conserved positions of OCT1 are strong predictors of decreased function and suggest that a combination of evolutionary conservation and chemical change might be a stronger predictor of function.
MeSH Terms
Alleles
Amino Acid Sequence
Animals
Cell Line
Conserved Sequence
Dogs
Evolution, Molecular
Gene Frequency
Genetic Variation
Humans
Kidney
Microscopy, Confocal
Models, Molecular
Molecular Sequence Data
Organic Cation Transporter 1/chemistry,genetics,metabolism
Protein Structure, Secondary
Recombinant Proteins/chemistry,metabolism
Transfection
Chemicals
Organic Cation Transporter 1
Recombinant Proteins
Authors & Affiliations
14 authors, click to expand affiliations / ORCID
Shu Yan
Department of Biopharmaceutical Sciences, Division of Clinical Pharmacology and Experimental Therapeutics, University of California, San Francisco, CA 94143-0446, USA.
Leabman Maya K
Feng Bo
Mangravite Lara M
Huang Conrad C
Stryke Doug
Kawamoto Michiko
Johns Susan J
DeYoung Joseph
Carlson Elaine
Ferrin Thomas E
Herskowitz Ira
Giacomini Kathleen M
Pharmacogenetics Of Membrane Transporters Investigators
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