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PMID: 12704234 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A sensitive, versatile microfluidic assay for bacterial chemotaxis.

Mao H, Cremer PS, Manson MD

Abstract

We have developed a microfluidic assay for bacterial chemotaxis in which a gradient of chemoeffectors is established inside a microchannel via diffusion between parallel streams of liquid in laminar flow. The random motility and chemotactic responses to L-aspartate, L-serine, L-leucine, and Ni(2+) of WT and chemotactic-mutant strains of Escherichia coli were measured. Migration of the cells was quantified by counting the cells accumulating in each of 22 outlet ports. The sensitivity of the assay is attested to by the significant response of WT cells to 3.2 nM L-aspartate, a concentration three orders of magnitude lower than the detection limit in the standard capillary assay. The response to repellents was as robust and easily recorded as the attractant response. A surprising discovery was that L-leucine is sensed by Tar as an attractant at low concentrations and by Tsr as a repellent at higher concentrations. This assay offers superior performance and convenience relative to the existing assays to measure bacterial tactic responses, and it is flexible enough to be used in a wide range of different applications.

MeSH Terms
Chemotaxis Escherichia coli/physiology
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mao Hanbin
Department of Chemistry, Texas A&M University, College Station, TX 77843, USA.
Cremer Paul S
Manson Michael D
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
2003-04-29
Epub
2003-00-18
Pages
5449-54
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC154365
Subset
IM
Grants
NIGMS NIH HHS · R01 GM039736 · United States
NIGMS NIH HHS · GM39736 · United States
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